Low levels of exogenous histone H1 in yeast cause cell death.
Low levels of exogenous histone H1 in yeast cause cell death.
复制标题
酵母中低水平的外源组蛋白 H1 会导致细胞死亡。
DOI：
10.1073/pnas.91.24.11567
复制
发表时间：
1994
影响因子：
11.1
通讯作者：
Zlatanova,J
中科院分区：
文献类型：
--
作者：
Miloshev,G;Venkov,P;vanHolde,K;Zlatanova,J
To elucidate the function of lysine-rich histone, yeast cells, which are believed to lack this histone, were transformed with an expression vector carrying the sea urchin histone H1 gene under control of an inducible promoter. Expression of full-length protein was tested by immunoblotting and the intracellular distribution was monitored by immunoelectron microscopy. Even low amounts of exogenous H1 led to dramatic changes in intracellular morphology and cell death. The cells that survived had lost either the plasmid or the ability to express the exogenous protein. Thus, even low amounts of canonical histone H1 are lethal to yeast cells.
