Nanoparticle delivery systems formed using electrically sprayed co-flowing excipients and active agent.
Nanoparticle delivery systems formed using electrically sprayed co-flowing excipients and active agent.
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DOI：
10.1166/jbn.2011.1353
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发表时间：
2011-12
影响因子：
2.9
通讯作者：
R. Bakhshi;Z. Ahmad;M. Soric;E. Stride;M. Edirisinghe
中科院分区：
文献类型：
--
作者：
R. Bakhshi;Z. Ahmad;M. Soric;E. Stride;M. Edirisinghe
载药生物可降解纳米颗粒的高生产率是实现纳米医学重大和快速进步的关键因素。在本文中，我们报告了一个一步，高产率，同轴电流体动力喷雾法产生胰岛素负载的聚合物纳米粒子。聚（乳酸-羟基乙酸）共聚物（PLGA）被用作模型生物降解聚合物。使用电子和原子力显微镜研究了颗粒形状和尺寸分布，并进行了长达五周的降解和体外胰岛素释放研究。通过简单的工艺控制变量（流速、施加电压和收集点）的操作，在环境温度下以80 +/-4.1%的包封效率生产小至50 nm的纳米颗粒。在喷雾时，产生了负载胰岛素的PLGA纳米颗粒，而不破坏胰岛素结构。通过使用少量的生物相容性亲水性添加剂，胰岛素突释在第一天和第二天分别减少20%和15%，并且随后在3周内相对持续释放。同轴电流体动力喷雾显示在单个步骤中添加和不添加赋形剂的情况下产生负载胰岛素的PLGA;在2重量%的聚合物浓度下，85%的颗粒<100 nm。这些负载胰岛素的PLGA纳米颗粒具有在3周内维持药物释放水平的潜力。
The high production rate generation of drug-loaded biodegradable nanoparticles is a crucial factor in achieving significant and rapid advances in nanomedicine. In this paper, we report a one-step, high yield, coaxial electrohydrodynamic spraying method for generating insulin-loaded polymeric nanoparticles. Poly(lactic-co-glycolic acid) copolymer (PLGA) was used as the model biodegradable polymer. The particle shape and size-distribution were studied using electron and atomic force microscopy and were also subjected to degradation and in vitro insulin release studies for up to five weeks. Nanoparticles as small as 50 nm were produced at the ambient temperature with an encapsulation efficiency of 80 +/- 4.1%, through manipulation of simple process control variables (flow rate, applied voltage and collection point). On spraying, insulin-loaded PLGA nanoparticles were generated without disruption to the insulin structure. By using small amounts of a biocompatible hydrophilic additive, the insulin burst release was reduced by 20% and 15% on the first and second days, respectively, and this was followed by comparatively sustained release over 3 weeks. Coaxial electrohydrodynamic spraying was shown to produce insulin-loaded PLGA, with and without the addition of excipients in a single step; with 85% of particles <100 nm at a polymer concentration of 2 wt%. These insulin-loaded PLGA nanoparticles have the potential to maintain the level of drug release over 3 weeks.