Tol-Pal proteins are critical cell envelope components of Erwinia chrysanthemi affecting cell morphology and virulence
Tol-Pal proteins are critical cell envelope components of Erwinia chrysanthemi affecting cell morphology and virulence
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DOI：
10.1099/mic.0.28237-0
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发表时间：
2005-10-01
期刊：
影响因子：
2.8
通讯作者：
Lazzaroni, JC
中科院分区：
文献类型：
--
作者：
Dubuisson, JF;Vianney, A;Lazzaroni, JC
tol-pal基因是维持革兰氏阴性菌外膜完整性所必需的。这些基因首先在大肠杆菌中被描述，最近在其他几个物种中被描述。它们参与大肠杆菌、杜克雷嗜血杆菌、霍乱弧菌和肠道沙门氏菌的致病过程。tol-pal基因在细菌致病性中的作用在植物病原性肠杆菌欧文氏菌中进行了研究，假设这种生物体可能是这种研究的良好模型。整个呃。对乔依多勒-帕尔地区进行了定性。除托拉外，Tol-C蛋白与它们的大肠杆菌同源物显示出高的同一性分数。儿通过在ybgC、tolQ、tolA、tolB、pal和ybgF基因中引入uidA-kan盒来构建Chaeni突变体。所有的突变体都对胆盐过敏。tolQ、托拉、tolB和pal中的突变对细菌是有害的，这需要高浓度的糖或糖保护剂来维持其活力。与这一观察结果一致，它们的细胞形态和分裂大大受损，这通过观察到细胞丝、球形、膜起泡和错误定位的细菌隔膜来证明。此外，tol-pal突变体在马铃薯块茎模型和菊苣叶上显示出降低的毒力。这可能是由于tol-pal突变体的表型受损，如生长和运动能力降低，以及果胶酸裂解酶（Er的主要毒力因子）的产生减少。查里岛
The tol-pal genes are necessary for maintaining the outer-membrane integrity of Gram-negative bacteria. These genes were first described in Escherichia coli, and more recently in several other species. They are involved in the pathogenesis of E coli, Haemophilus ducreyi, Vibrio cholerae and Salmonella enterica. The role of the tol-pal genes in bacterial pathogenesis was investigated in the phytopathogenic enterobacterium Erwinia chrysanthemi, assuming that this organism might be a good model for such a study. The whole Er. chrysanthemi tol-pal region was characterized. Tol-Pal proteins, except TolA, showed high identity scores with their E coli homologues. Er. chrysanthemi mutants were constructed by introducing a uidA-kan cassette in the ybgC, tolQ, tolA, tolB, pal and ybgF genes. All the mutants were hypersensitive to bile salts. Mutations in tolQ, tolA, tolB and pal were deleterious for the bacteria, which required high concentrations of sugars or osmoprotectants for their viability. Consistent with this observation, they were greatly impaired in their cell morphology and division, which was evidenced by observations of cell filaments, spherical forms, membrane blebbing and mislocalized bacterial septa. Moreover, tol-pal mutants showed a reduced virulence in a potato tuber model and on chicory leaves. This could be explained by a combination of impaired phenotypes in the tol-pal mutants, such as reduced growth and motility and a decreased production of pectate lyases, the major virulence factor of Er. chrysanthemi.