Cyanobacterial light-harvesting complex subunits encoded in two red light-induced transcripts.
Cyanobacterial light-harvesting complex subunits encoded in two red light-induced transcripts.
复制标题
蓝藻光捕获复合物亚基编码在两个红光诱导的转录本中。
DOI：
10.1126/science.3931221
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Grossman,AR
中科院分区：
文献类型：
--
作者：
Conley,PB;Lemaux,PG;Grossman,AR
蓝藻和红藻的主要捕光复合体--藻胆体由发色多肽和非发色多肽组成。从蓝藻Fremyella diplosiphon中分离到两个编码主要发色成分的连锁基因，即藻蓝蛋白的多肽亚基。这个藻蓝蛋白亚单位基因簇的转录本作为主要物种存在于红光下生长的蓝藻中，而不存在于绿光下培养的蓝藻中。红光诱导藻蓝蛋白各亚单位的基因在两种信使核糖核酸上转录(β-藻蓝蛋白和α-藻蓝蛋白)，一个有1600个碱基，另一个有3800个碱基。后者包含较小的转录本，包含从α-藻蓝蛋白基因编码区3‘端延伸的附加序列。它可以编码藻胆体的其他光诱导成分。由于藻蓝蛋白有效地吸收红光，成为红光下藻胆体的主要成分，这些不同的水平可能反映了这些生物对环境的一种重要的适应机制。
The major light-harvesting complex in cyanobacteria and red algae, the phycobilisome, is composed of chromophoric and nonchromophoric polypeptides. Two linked genes encoding major chromophoric components, the polypeptide subunits of phycocyanin, were isolated from the cyanobacteriumFremyella diplosiphon. Transcripts from this phycocyanin subunit gene cluster were present as major species in the cyanobacterium grown in red light, but not in cultures maintained in green light. The genes for the subunits of the red light-induced phycocyanin were transcribed together (β-phycocyanin followed by α-phycocyanin) on two messenger RNA species; one contained 1600 bases while the other had 3800 bases. The latter, which encompassed the smaller transcript, contained additional sequences extending from the 3′ end of the coding region of the α-phycocyanin gene. It may encode other light-induced components of the phycobilisome. Since phycocyanin, which effectively absorbs red light, becomes a dominant constituent of the phycobilisome in red light, these different levels may reflect an important adaptive mechanism of these organisms to their environment.