Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding
Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding
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DOI：
10.1210/en.2007-1276
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发表时间：
2008-03-01
期刊：
影响因子：
4.8
通讯作者：
Yada, Toshihiko
中科院分区：
文献类型：
--
作者：
Kohno, Daisuke;Nakata, Masanori;Yada, Toshihiko
Nesfatin-1是一种新发现的饱腹感分子，位于下丘脑室旁核和视上核。本研究采用神经肽和c-Fos免疫组织化学方法，对nesfatin-1神经元在PVN和SON的精细定位和调控进行了研究。在PVN中，24%的nesfatin-1神经元与催产素重叠，18%与加压素重叠，13%与CRH重叠，12%与TRH神经元重叠。在SON中，35%的nesfatin-1神经元与催产素重叠，28%与加压素重叠。禁食48小时后，再喂食2小时，与禁食对照组相比，室旁核中表达c-Fos免疫反应性的nesfatin-1神经元的数量显著增加了约10倍，SON中增加了30倍。在SON中，与禁食相比，再喂养也显著增加nesfatin-1免疫反应神经元的数量和NUCB 2 mRNA的表达。这些结果表明，nesfatin-1神经元在PVN和SON高度重叠的催产素和加压素神经元，它们被激活显着再喂养。摄食激活的下丘脑室旁核和视上核nesfatin-1神经元可能在摄食行为和能量稳态的餐后调节中发挥作用。
Nesfatin-1, a newly discovered satiety molecule, is located in the hypothalamic nuclei, including the paraventricular nucleus (PVN) and supraoptic nucleus (SON). In this study, fine localization and regulation of nesfatin-1 neurons in the PVN and SON were investigated by immunnhistochemistry of neuropeptides and c-Fos. In the PVN, 24% of nesfatin-1 neurons overlapped with oxytocin, 18% with vasopressin, 13% with CRH, and 12% with TRH neurons. In the SON, 35% of nesfatin-1 neurons overlapped with oxytocin and 28% with vasopressin. After a 48-h fast, refeeding for 2 h dramatically increased the number of nesfatin-1 neurons expressing c-Fos immunoreactivity by approximately 10 times in the PVN and 30 times in the SON, compared with the fasting controls. In the SON, refeeding also significantly increased the number of nesfatin-1-immunoreactive neurons and NUCB2 mRNA expression, compared with fasting. These results indicate that nesfatin-1 neurons in the PVN and SON highly overlap with oxytocin and vasopressin neurons and that they are activated markedly by refeeding. Feeding-activated nesfatin-1 neurons in the PVN and SON could play a role in the postprandial regulation of feeding behavior and energy homeostasis.