Cupular organs in two species of Corella (Tunicata: Ascidiacea)
Cupular organs in two species of Corella (Tunicata: Ascidiacea)
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两种 Corella 物种的杯状器官（被囊目：Ascidiacea）
DOI：
10.1111/j.1744-7410.2004.tb00161.x
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发表时间：
2005
影响因子：
1.2
通讯作者：
C. L. Singla
中科院分区：
文献类型：
--
作者：
G. Mackie;C. L. Singla
.在科雷利亚真肌中观察到与玻璃海鞘中描述的相似的简单杯状器官。它们由一个包含20-30个初级感觉神经元的细胞体的黄斑组成，这些初级感觉神经元的纤毛突出成圆顶状或指状结构，即杯状结构。而不是像C.在肠内，这些器官位于鳃囊的心房表面。感觉神经支配进行了检查，在整装准备使用抗微管蛋白免疫组织化学。C. eumyota没有表现出免疫反应性的抗血清对促性腺激素释放激素（GnRH）。
在膨胀科雷利亚中发现了一种新的细长的感觉器官，称为杯状股。它具有与简单型杯状器相同的基本组成部分，但由一个单一的长形结构组成，包含约1500个感觉细胞。位于鳃囊的心房表面，它沿着背褶的中线延伸，从性腺管开口几乎一直延伸到大脑。使用光学和电子显微镜检查制剂。通过抗微管蛋白免疫荧光显微镜观察神经和纤毛。我们有可能追踪感觉轴突从杯状束的黄斑到它们连接内脏神经分支的点，内脏神经进入大脑后部的神经根。
In C.肿胀的感觉细胞体和它们的轴突的免疫反应不仅与抗微管蛋白，但也与抗血清提出的被囊I GnRH。然而，鸡II和鲶鱼GnRH抗血清没有免疫反应性。所有三种GnRH抗血清都标记了背束丛，这是一种与GnRH作为生殖激素的作用有关的结构。我们的结论是，感觉神经元中标记的GnRH样分子与背束神经丛中发现的GnRH形式不同，并且可能具有不同的功能，可能是在纤毛活动的神经控制中。
在科雷利亚物种的杯状器官的功能尚未进行生理学研究，但通过与其他后生动物中的这种结构进行类比，杯状器官可能是记录通过心房腔的水流的局部扰动或变化的流体动力学传感器。
. Simple cupular organs similar to those described in Ciona intestinalis were observed in Corella eumyota. They consist of a macula containing the cell bodies of 20–30 primary sensory neurons whose cilia project into a dome- or finger-shaped structure, the cupula. Rather than being found in the mantle lining as in C. intestinalis, the organs were located on the atrial surface of the branchial sac. The sensory innervation was examined in whole-mount preparations using anti-tubulin immunohistochemistry. Sensory neurons in C. eumyota showed no immunoreactivity with antisera raised against gonadotropin-releasing hormone (GnRH).
A novel, elongated sense organ termed the cupular strand was found in Corella inflata. It has the same basic components as the simple type of cupular organ but consists of a single, long structure containing ∼1500 sensory cells. Located on the atrial surface of the branchial sac, it extends along the midline of the dorsal fold, from the gonoduct openings almost as far as the brain. Preparations were examined using optical and electron microscopy. Nerves and cilia were visualized by anti-tubulin immunofluorescence microscopy. It was possible to follow the sensory axons from the macula of the cupular strand to points where they joined branches of the visceral nerve, which enters a nerve root at the back of the brain.
In C. inflata the sensory cell bodies and their axons were immunoreactive not only with anti-tubulin but also with an antiserum raised against Tunicate I GnRH. There was no immunoreactivity, however, with Chicken II and catfish GnRH antisera. All three GnRH antisera labeled the dorsal strand plexus, a structure associated with production of GnRH in its role as a reproductive hormone. We concluded that the GnRH-like molecule labeled in sensory neurons differs from the form of GnRH found in the dorsal strand plexus, and may have a different function, perhaps in the neural control of ciliary activity.
The function of the cupular organs in species of Corella has not yet been investigated physiologically, but by analogy with such structures in other metazoans, cupular organs are probably hydrodynamic sensors registering local disturbances or changes in water flow through the atrial cavity.
影响因子：
4.8
作者：
Adams, BA;Tello, JA;Sherwood, NM
通讯作者：
Sherwood, NM