Expression of human erythropoietin transgenes and of the endogenous wap gene in the mammary gland of transgenic rabbits during gestation and lactation
Expression of human erythropoietin transgenes and of the endogenous wap gene in the mammary gland of transgenic rabbits during gestation and lactation
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DOI：
10.1023/a:1008882332133
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发表时间：
1998-07-01
影响因子：
3
通讯作者：
Castro, FO
中科院分区：
文献类型：
--
作者：
Aguirre, A;Castro-Palomino, N;Castro, FO
了解转基因在妊娠期和哺乳期乳腺中的表达对于使用转基因哺乳动物作为生物反应器至关重要。在这里，我们描述了兔WAP启动子和3'侧翼序列控制下的内源性兔WAP基因和人促红细胞生成素（hEPO）转基因表达的时间模式。内源性兔WAP基因在整个妊娠期表达，包括交配日，以及在哺乳期携带微基因的转基因兔;构建体。在非怀孕的骑自行车的女性，WAP表达被发现独立的转基因状态，然而，WAP表达未检测到非骑自行车的女性。这一新发现的意义目前尚不清楚，hEPO mRNA仅在妊娠第28天的妊娠转基因兔乳腺活检中检测到。在哺乳期间，转录本存在于第0、7、14和21天采集的乳腺活检样品中。在哺乳期结束时（第28天），发现hEPOcDNA基因构建体的转录物水平急剧下降。虽然hEPO的水平太低，无法得出关于时间或位置依赖性表达的结论，但这一发现可能反映了整合位置效应。
An understanding of the expression of transgenes in the mammary gland during gestation and lactation is crucial for the use of transgenic mammals as bioreactors. Here we describe the temporal pattern of expression of the endogenous rabbit WAP gene and human erythropoietin (hEPO) transgenes under the control of rabbit WAP promoter and 3' flanking sequences. The endogenous rabbit WAP gene was expressed throughout gestation including the day of mating, as well as during lactation in transgenic rabbits bearing a minigene;construct. In nonpregnant cycling females, WAP expression was found independent of transgenic status, however, WAP expression was not detected in non-cycling females. The significance of this new finding is not clear at present, hEPO mRNA was detected in mammary gland biopsies from pregnant transgenic rabbits only on day 28 of gestation. During lactation, transcripts were present in mammary gland biopsy samples taken on days 0, 7, 14 and 21. A sharp decline in the levels of transcripts was found for an hEPOcDNA gene construct at the end of lactation (day 28). Although the levels of hEPO were too low to allow a conclusion to be drawn regarding temporal or position-dependent expression, this finding may reflect an integration position effect.