Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles
Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles
复制标题
Salecan 通过调节 T 细胞免疫反应和基于 NMR 的代谢谱来预防刀豆蛋白 A 诱导的急性肝损伤
DOI：
10.1016/j.taap.2017.01.007
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发表时间：
2017-02-15
影响因子：
3.8
通讯作者：
Zhang, Jianfa
中科院分区：
文献类型：
--
作者：
Sun, Qi;Xu, Xi;Zhang, Jianfa
Salecan是由农杆菌sp. ZX09生产的一种水溶性胞外β -葡聚糖，据报道具有广泛的生物学效应。本研究的目的是研究salecan对ConA （ConA）诱导的肝炎（免疫介导的肝损伤动物模型）的保护作用，并寻找可能的机制。C57BL/6小鼠经salecan预处理后再注射ConA。Salecan治疗可显著减轻cona诱导的急性肝损伤，抑制cona诱导的肝损伤模型中炎性细胞因子干扰素(IFN)- γ、白细胞介素(IL)-6、IL-1 β的表达和分泌。经salecan处理后，ConA诱导的肝脏中MIP-1 α、MIP-1 β、ICAM-1、MCP-1、RANTES等趋化因子和粘附分子的高表达水平也有所下调。Salecan可抑制ConA诱导的肝脏炎症细胞，尤其是T细胞的浸润和活化。此外，salecan通过部分恢复ConA引起的代谢紊乱，使ConA治疗小鼠的代谢谱向对照组逆转。我们的研究结果提示salecan在免疫介导性肝炎中的预防和治疗潜力。(C) 2017爱思唯尔公司版权所有。
Salecan, a water-soluble extracellular beta-glucan produced by Agrobacterium sp. ZX09, has been reported to exhibit a wide range of biological effects. The aims of the present study were to investigate the protective effect of salecan against Concanavalin A (ConA)-induced hepatitis, a well-established animal model of immune-mediated liver injury, and to search for possible mechanisms. C57BL/6 mice were pretreated with salecan followed by ConA injection. Salecan treatment significantly reduced ConA-induced acute liver injury, and suppressed the expression and secretion of inflammatory cytokines including interferon (IFN)-gamma, interleukin (IL)-6 and IL-1 beta in ConA-induced liver injury model. The high expression levels of chemokines and adhesion molecules such as MIP-1 alpha, MIP-1 beta, ICAM-1, MCP-1 and RANTES in the liver induced by ConA were also down-regulated after salecan treatment. Salecan inhibited the infiltration and activation of inflammatory cells, especially T cells, in the liver induced by ConA. Moreover, salecan reversed the metabolic profiles of ConA-treated mice towards the control group by partly recovering the metabolic perturbations induced by ConA. Our results suggest the preventive and therapeutic potential of salecan in immune-mediated hepatitis. (C) 2017 Elsevier Inc. All rights reserved.