IDENTIFICATION OF MIM23, A PUTATIVE COMPONENT OF THE PROTEIN IMPORT MACHINERY OF THE MITOCHONDRIAL INNER MEMBRANE
IDENTIFICATION OF MIM23, A PUTATIVE COMPONENT OF THE PROTEIN IMPORT MACHINERY OF THE MITOCHONDRIAL INNER MEMBRANE
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DOI：
10.1016/0014-5793(93)80921-g
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发表时间：
1993-09-06
期刊：
影响因子：
3.5
通讯作者：
MEIJER, M
中科院分区：
文献类型：
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作者：
DEKKER, PJT;KEIL, P;MEIJER, M
筛选线粒体蛋白质进口受损的酵母突变体导致鉴定了编码内膜进口机器的基本组分MIM44和MIM17的两个基因（MPI1和MPI2）。我们分析了筛选中获得的另外12个突变体，并发现了另外两个互补组。一组代表SSC1的突变体，SSC1是编码线粒体hsp70的基因，hsp70是一种必需的基质蛋白，是蛋白质跨内膜输入所需的。第二互补组代表编码23 kDa整合内膜蛋白（MIM23）的新基因（MPI3）的突变体。MIM23是在没有前序列的情况下合成的，并且其向内膜的输入需要膜电位。MIM23含有与MIM17的一半同源的结构域。我们推测MIM23是线粒体内膜蛋白质输入机制的新成员。
A screening for yeast mutants impaired in mitochondrial protein import led to the identification of two genes (MPI1 and MPI2) encoding the essential components MIM44 and MIM17 of the inner membrane import machinery. We analyzed twelve additional mutants obtained in the screening and found two further complementation groups. One group represents mutants of SSC1, the gene encoding mitochondrial hsp70, an essential matrix protein required for protein import across the inner membrane. The second complementation group represents mutants of a new gene (MPI3) encoding a 23 kDa integral inner membrane protein (MIM23). MIM23 is synthesized without a presequence, and its import to the inner membrane requires a membrane potential. MIM23 contains a domain homologous to half of MIM17. We speculate that MIM23 is a new member of the protein import machinery of the mitochondrial inner membrane.