Rapid determination of short-chain fatty acids in colonic contents and faeces of humans and rats by acidified water-extraction and direct-injection gas chromatography
Rapid determination of short-chain fatty acids in colonic contents and faeces of humans and rats by acidified water-extraction and direct-injection gas chromatography
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DOI：
10.1002/bmc.580
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发表时间：
2006-08-01
影响因子：
1.8
通讯作者：
Jonsson, Jan Ake
中科院分区：
文献类型：
--
作者：
Zhao, Guohua;Nyman, Margareta;Jonsson, Jan Ake
短链脂肪酸（SCFAs）因其积极的生理作用而受到广泛关注。在这项工作中，一个快速和可靠的气相色谱法测定8 SCFAs，从大鼠和人类的结肠和粪便样品已被开发和验证。该方法包括在FFAP毛细管柱上直接注射程序之前在水中提取SCFAs。制备并使用含有乙酸、丙酸、正丁酸、异丁酸、正戊酸、异戊酸、正己酸和正庚酸的标准品贮备液。该方法线性关系好（r（2）> 0.9990），定量限低（2.38-30.14 μ M），回收率高。发现粪便样品的酸化对于SCFAs的定量测定至关重要，并且认为将pH调节至2-3是必要的。在进样口的玻璃内衬中插入玻璃棉被证明可以有效地防止非挥发性物质对色谱柱的污染，12%甲酸可以降低多次进样后逐渐出现的鬼峰。验证后，该方法被应用于两个粪便样品从大鼠喂养的饮食含有不同量的膳食纤维和一个粪便样品从人类喂养的正常饮食，以测试开发的方法的准确性。版权所有（c）2005年约翰威利父子有限公司。
Short-chain fatty acids (SCFAs) have attracted much attention recently because of their positive physiological effects. In this work, a rapid and reliable gas chromatographic method for determination of eight SCFAs, in colonic and faecal samples from rats and humans has been developed and validated. The methodology involves extraction of the SCFAs in water before a direct injection procedure on a FFAP capillary column. A stock standard solution containing acetic acid, propionic acid, n-butyric acid, i-butyric acid, n-valeric acid, i-valeric acid, n-caproic acid and n-heptanoic acid was prepared and used. A high linearity (r(2) > 0.9990), low quantification limit (2.38-30.14 mu M) and high recovery for most acids were obtained. Acidification of faecal samples was found to be crucial for quantitative determination of the SCFAs, and adjustment of pH to 2-3 was regarded as necessary. Glass wool inserted in the glass liner of the injection port proved effective in preventing the contamination of the column by non-volatiles, and 12% formic acid reduced the ghost peak that appeared gradually after several injections. After validation, the methodology was applied on two faecal samples from rats fed diets containing different amount of dietary fibre and one faecal sample from human fed a normal diet to test the accuracy of the developed method. Copyright (c) 2005 John Wiley & Sons, Ltd.