Reproductive toxicity of 2-bromopropane in Sprague Dawley rats
Reproductive toxicity of 2-bromopropane in Sprague Dawley rats
复制标题
2-溴丙烷对 Sprague Dawley 大鼠的生殖毒性
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Moon Yh
中科院分区：
文献类型：
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作者：
Y. Ij;Chung Yh;Lim Ch;Maeng Sh;Lee Jy;Kim Hy;Lee Sj;Kim Ch;Kim Tg;Parks Js;Moon Yh
目的为了澄清韩国某电子公司工人的集体中毒事件，对可能导致生殖毒性的化学物质2-溴丙烷（2BP）进行了调查。方法采用雄性Sprague道利大鼠重复给药法测定2 BP。将10只大鼠分配至每个处理组。连续28天腹膜内注射溶剂对照橄榄油和浓度为125 mg/kg、250 mg/kg和500 mg/kg的2BP。结果2BP剂量越大，大鼠体重越轻。根据2BP的剂量，右侧和左侧睾丸显示出典型的体重减轻。红细胞、血红蛋白和红细胞压积在高剂量下出现一定程度的下降。血红蛋白量、平均血小板体积、白色细胞数和淋巴细胞数随2BP剂量增加而显著减少，而粒细胞和单核细胞数则有随2BP剂量增加而减少的趋势。用中等和高剂量2BP处理的睾丸的组织病理学显示典型的斑片外观，具有严重耗尽的萎缩小管，表现出生精小管中的精原细胞和精母细胞的生殖细胞坏死。间质组织中的间质细胞增生或肥大也很明显。附睾出现一定程度的萎缩，伴附睾上皮空泡化。结论睾丸是2BP毒性的主要靶器官。2BP还影响造血系统，从而引起白细胞减少和正常红细胞贫血。除生殖器官和造血系统外，未发现显著毒性。
Objectives In an effort to clarify the mass intoxication of workers at an electronic company in Korea, the possible causative chemical for reproductive toxicity, 2-bromopropane (2BP), was investigated. Methods 2BP was tested through the use of repeated dose experiments among male Sprague Dawley rats. Ten rats were assigned to each treatment group. Vehicle control olive oil and 2BP concentrations of 125 mg/kg, 250 mg/kg, and 500 mg/kg were injected into the intraperitoneum on 28 consecutive days. Results The rats showed significant decreases in body weight depending on the 2BP dose. The right and left testes showed typical weight loss depending on the dose of 2BP. The red blood cells, hemoglobin, and hematocrit showed some degree of decline with the high dose. The amount of hemoglobin, the mean platelet volume, the number of white blood cells, and the number of lymphocytes decreased significantly with the high dose, while the number of granulocytes and monocytes had a tendency to decrease depending on the dose of 2BP. The histopathology of the testes treated with the middle and high 2BP dose showed a typical patch appearance with severely depleted atrophic tubules, exhibiting germ cell necrosis of spermatogonia and spermatocytes in the seminiferous tubules. Leydig cell hyperplasia or hypertrophy in the interstitial tissue was also noticeable. The epididymis showed some degree of atrophy with vacuolization of the epididymal epithelium. Conclusion The testes are the main target organs tested for 2BP toxicity. 2BP also affect the hematopoietic system and thus induce leukopenia and normocytic anemia. Besides the reproductive organs and the hematopoietic system, no significant toxicity has been found.