Antifibrotic potential of bone marrow-derived mesenchymal stem cells in biliary atresia mice
Antifibrotic potential of bone marrow-derived mesenchymal stem cells in biliary atresia mice
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胆道闭锁小鼠骨髓间充质干细胞的抗纤维化潜力
DOI：
10.3892/mmr.2018.9353
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发表时间：
2018
影响因子：
3.4
通讯作者：
Zhang Shouhua
中科院分区：
文献类型：
--
作者：
Lei Jun;Chai Yong;Xiao Juhua;Hu Huakun;Liu Zhiqiang;Xiao Yu;Yi Lijun;Huang Jinshi;Xiang Tianxin;Zhang Shouhua
胆道闭锁（BA）是一种罕见的严重疾病，影响婴儿，其中纤维炎症过程破坏胆管，导致纤维化和胆汁性肝硬化，如果不治疗会导致死亡。骨髓间充质干细胞（BMMSCs）被认为是一种有前途的治疗纤维化疾病的方法。本研究的目的是探讨骨髓间充质干细胞移植在BA小鼠模型中的抗纤维化作用。通过对新生小鼠接种恒河猴轮状病毒建立小鼠BA模型。结果表明，BA模型组大鼠肝酶和胆红素代谢水平、氧化应激指标丙二醛（MDA）和肝纤维化指标均升高，而肝组织超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH）水平降低。苏木精-伊红和Masson三色染色显示BA肝脏中存在严重的肝纤维化和胶原积聚。然而，一旦BA小鼠接种BMMSC，这些指标均逆转。总之，本研究证明了BMMSCs在BA小鼠中的抗纤维化潜力，这可能为改善BA患者的纤维化反应提供新的方法。
Biliary atresia (BA) is a rare and severe disease that affects infants where a fibroinflammatory process destroys the bile ducts, leading to fibrosis and biliary cirrhosis, and mortality if untreated. Bone marrow-derived mesenchymal stem cells (BMMSCs) have been considered as a promising therapy in fibrotic diseases. The aim of the present was to investigate the anti-fibrotic roles of BMMSC transplantation in a BA mouse model. Mouse BA models were established by Rhesus rotavirus administration to neonatal mice. The results revealed that the liver enzyme and bilirubin metabolism levels, and the levels of the oxidative stress marker malondialdehyde (MDA) and the fibrosis marker were all increased in the BA model, while the liver tissue levels of superoxide dismutase and glutathione peroxidase were reduced. The hematoxylin and eosin and Masson's trichrome staining revealed severe liver fibrosis and collagen accumulation in BA livers. However, these indicators were all reversed once the BA mice were administered the BMMSC inoculation. In conclusion, the present study demonstrated the anti-fibrotic potential of BMMSCs in BA mice, which may provide a novel approach to ameliorate the fibrotic response in BA patients.