Genetic differences in cocaine-induced conditioned place preference in mice depend on conditioning trial duration
Genetic differences in cocaine-induced conditioned place preference in mice depend on conditioning trial duration
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DOI：
10.1007/s002130051090
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
McMullin, CS
中科院分区：
文献类型：
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作者：
Cunningham, CL;Dickinson, SD;McMullin, CS
理由：在之前与 C57BL/6J 小鼠的比较中，DBA/2J 小鼠被描述为在条件性位置偏好范式中对可卡因的奖赏效应“反应低下”。这一发现与其他研究形成鲜明对比，其他研究显示 DBA/2J 小鼠对位置调节任务中乙醇和吗啡的奖励作用更敏感。目的：本研究的目的是使用类似于之前评估乙醇和吗啡偏好调节的装置和程序来检查两种菌株中可卡因诱导的位置调节。方法：两种品系的小鼠均接受使用 1、10 或 30 毫克/千克可卡因的无偏位置调节程序。调节试验持续时间为 15、30 或 60 分钟。结果：一般来说，C57BL/6J 小鼠表现出显着的条件性位置偏好，相对不受可卡因剂量或试验持续时间的影响。相比之下，DBA/2J 小鼠在最短的试验持续时间内没有表现出位置调节，但随着试验持续时间的增加，偏好程度也随之增加。在最长的试验持续时间内，两种菌株都表现出相似的位置偏好水平。结论：对可卡因奖赏效应敏感性的遗传差异主要取决于位置调节过程的时间参数。对这些发现的一种可能的解释是，较短的试验持续时间会产生条件性活动反应，与 C57BL/6J 小鼠相比，DBA/2J 小鼠对条件性位置偏好表达的干扰更大。更一般地说，这些发现强调在得出关于位置调节的遗传差异的结论时需要谨慎，特别是在使用这种范式来评估基因敲除或插入对药物奖励的影响时。
Rationale: In previous comparisons with C57BL/6J mice, DBA/2J mice have been characterized as "hyporesponsive" to cocaine's rewarding effect in the conditioned place-preference paradigm. This finding contrasts with other studies showing greater sensitivity of DBA/2J mice to the rewarding effects of ethanol and morphine in the place conditioning task. Objectives: The purpose of the present study was to examine cocaine-induced place conditioning in both strains using apparatus and procedures similar to those used previously to assess ethanol and morphine preference conditioning. Methods: Mice from both strains were exposed to an unbiased place-conditioning procedure using 1, 10, or 30 mg/kg cocaine. Conditioning trial duration was 15, 30, or 60 min. Results: In general, C57BL/6J mice displayed a significant conditioned place preference that was relatively unaffected by cocaine dose or trial duration. In contrast, DBA/2J mice showed no place conditioning at the shortest trial duration, but an increasing level of preference as trial duration increased. At the longest trial duration, both strains showed similar levels of place preference. Conclusions: Genetic differences in sensitivity to cocaine's rewarding effect depend critically on temporal parameters of the place-conditioning procedure. One possible interpretation of these findings is that short trial durations produce conditioned activity responses that interfere more with expression of conditioned place preference in DBA/2J mice than in C57BL/6J mice. More generally, these findings underscore the need for caution when drawing conclusions about genetic differences in place conditioning, especially when using this paradigm to evaluate the effects of gene knockouts or insertions on drug reward.