Studies on choline ad ethanolamine phosphoglyceride biosynthesis in developing rabbit brain.
Studies on choline ad ethanolamine phosphoglyceride biosynthesis in developing rabbit brain.
复制标题
兔脑发育过程中胆碱和乙醇胺磷酸甘油酯生物合成的研究。
DOI：
10.1002/jnr.490050508
复制
发表时间：
1980
影响因子：
4.2
通讯作者：
Percy,AK
中科院分区：
文献类型：
--
作者：
Fimbres,E;Saenz,R;Smith,N;Percy,AK
在兔大脑微粒体制备物中评价胆碱磷酸转移酶（EC 2.7.8.2）和乙醇胺磷酸转移酶（EC 2.7.8.1）的活性。动物的年龄范围从妊娠19天到产后发育2个月。在最佳条件下，这些转移酶在兔脑中的性质相对于pH值、二价阳离子需求以及CDP-中间体和脂质受体的表观Km值非常相似。胆碱磷酸转移酶特异性活性在胎儿生命的最后15%-20%期间急剧升高，并在出生后第15天附近第二次升高至最大值。乙醇胺磷酸转移酶比活性在同一时期表现出类似的，但不太明显的年龄相关性增加。在同一时期（妊娠24天至出生后第19天），兔大脑的生长（通过重量测定）增加了30倍。这些转移酶的特异性活性在中期胎兔和早期成年兔的脑制备物中相似。胆碱磷酸转移酶的最大比活性是乙醇胺磷酸转移酶的3-4倍，这些酶的比活性随年龄的变化似乎与妊娠最后1/3和出生后前3周兔大脑中发生的形态和组成事件的发育模式有关。
Activities of cholinephosphotransferase (EC 2.7.8.2) and ethanolaminephosphotransferase (EC 2.7.8.1) were evaluated in microsomal preparations derived from rabbit cerebrum. Animals ranged in age from nineteen day's gestation through two months of postnatal development. Under optimal conditions the properties of these transferases in rabbit brain were remarkably similar relative to pH, divalent cation requirements, and apparent‐Km values for the CDP‐intermediates and the lipid acceptor, respectively. Cholinephosphotransferase‐specific activity exhibited a dramatic rise during the last 15%–20% of fetal life and a second rise to a maximum near the 15th postnatal day. Ethanolaminephosphotransferase‐specific activity exhibited a similar, but less striking age‐related increase during the same period. Growth of rabbit cerebrum, as determined by weight, increased 30‐fold during this same period (24 days gestation to the 19th postnatal day). Specific activities of these transferases were similar in brain preparations from mid‐fetal and early adult rabbits. The maximum specific activity of cholinephosphotransferase exceeded that of ethanolaminephosphotransferase by 3–4‐fold.Age‐related changes in the specific activities of these transferases appear to correlate with the developmental pattern of morphological and compositional events occurring in rabbit cerebrum during the last third of gestation and the first three weeks of postnatal life.