Pseudomonas aeruginosa secreted factors impair biofilm development in Candida albicans
Pseudomonas aeruginosa secreted factors impair biofilm development in Candida albicans
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DOI：
10.1099/mic.0.037549-0
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发表时间：
2010-05-01
期刊：
影响因子：
2.8
通讯作者：
Morrissey, John P.
中科院分区：
文献类型：
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作者：
Holcombe, Lucy J.;McAlester, Gordon;Morrissey, John P.
人类机会致病菌铜绿假单胞菌和白色念珠菌之间的信号介导的相互作用影响这两种生物的毒力性状。表型研究表明，四种铜绿假单胞菌菌株的细菌上清液强烈降低了C.白色念珠菌在硅胶上形成生物膜。这在很大程度上是生物膜成熟的抑制的结果，这一现象也在从非临床细菌物种制备的上清液中观察到。上清液对生物膜形成的影响不通过干扰酵母-菌丝形态转换来介导，并且无论产生的高丝氨酸内酯（HSL）水平如何都发生，这表明该影响是HSL独立的。通过转录组分析来剖析铜绿假单胞菌上清液对C.白色念珠菌生物膜形成鉴定了238个基因，其响应于所有四种上清液表现出可重复的表达变化。特别是，与药物或毒素外排相关的基因表达强烈增加，与粘附和生物膜形成相关的基因表达减少。此外，编码已知抑制生物膜形成的蛋白质的YWP 1的表达显著增加。生物膜的形成是C.因此，铜绿假单胞菌拮抗这种感染的能力具有明确的生物医学意义。
Signal-mediated interactions between the human opportunistic pathogens Pseudomonas aeruginosa and Candida albicans affect virulence traits in both organisms. Phenotypic studies revealed that bacterial supernatant from four P. aeruginosa strains strongly reduced the ability of C. albicans to form biofilms on silicone. This was largely a consequence of inhibition of biofilm maturation, a phenomenon also observed with supernatant prepared from non-clinical bacterial species. The effects of supernatant on biofilm formation were not mediated via interference with the yeast-hyphal morphological switch and occurred regardless of the level of homoserine lactone (HSL) produced, indicating that the effect is HSL-independent. A transcriptome analysis to dissect the effects of the P. aeruginosa supernatants on gene expression in the early stages of C. albicans biofilm formation identified 238 genes that exhibited reproducible changes in expression in response to all four supernatants. In particular, there was a strong increase in the expression of genes related to drug or toxin efflux and a decrease in expression of genes associated with adhesion and biofilm formation. Furthermore, expression of YWP1, which encodes a protein known to inhibit biofilm formation, was significantly increased. Biofilm formation is a key aspect of C. albicans infections, therefore the capacity of P. aeruginosa to antagonize this has clear biomedical implications.