THE DEVELOPMENT AND PATTERN OF INNERVATION OF THE AVIAN CORNEA
THE DEVELOPMENT AND PATTERN OF INNERVATION OF THE AVIAN CORNEA
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DOI：
10.1016/0012-1606(82)90145-2
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
BEE, JA
中科院分区：
文献类型：
--
作者：
BEE, JA
鸟类角膜发育中神经的参与知之甚少，主要是因为角膜神经的演示一直难以捉摸。应用改良的Bodian染色技术证明角膜神经支配的发展。在胚胎发育的第6天，在眼周间充质内观察到许多大的轴突束到达角膜的腹颞侧。这些神经束分为2个不同的组，它们向腹侧迁移，并更广泛地向角膜周围的背侧迁移。角膜周围的神经束的渐进迁移发生在第7和第8天的发展和第10天的角膜内的神经环鞘。伴随着环的形成，观察到神经离开主要神经束并向角膜迁移。第12天，观察到许多神经突起，通过中间基质进入，向角膜中心迁移。在第12天检测到上皮的神经支配，从外周开始，并随着发育而急剧增加。神经支配的上皮细胞几乎是完整的第16天和神经的渗透到中央基质发生在第18天的发展。第16天，基底上皮细胞开始显示银染特性。这种染色的水平随着发育而增加，在孵化的鳞状细胞表现出特征性的银染色模式。未观察到角膜内皮的神经支配。鸟类角膜及其上皮细胞显然在同一发育期内受到神经支配，其中角膜从混浊到透明的主要转变是实现的。
The involvement of nerves in the development of the avian cornea is poorly understood, primarily because the demonstration of corneal nerves has been elusive. The development of corneal innervation was demonstrated by the application of a modified Bodian staining technique. On the 6th day of embryonic development, numerous large fascicles of axons were observed arriving at the ventrotemporal aspect of the cornea, within the periocular mesenchyme. These fascicles subdivided into 2 distinct groups which migrated both ventrally and, more extensively, dorsally around the cornea. Progressive migration of nerve fascicles around the cornea occurred through the 7th and 8th days of development and by the 10th day the cornea was ensheathed within a ring of nerves. Concomitant with ring formation, nerves were observed leaving the main nerve fascicles and migrating toward the cornea. Numerous nerve processes, which enter through the midstroma, were observed migrating toward the center of the 12th-day cornea. Innervation of the epithelium was detected on the 12th day, beginning at the periphery and increasing dramatically with development. Innervation of the epithelium was almost complete on the 16th day and penetration of nerves into the central stroma occurred on the 18th day of development. On the 16th day, the basal epithelial cells began to demonstrate Ag-staining properties. The levels of this staining increased with development and in the hatchling the squamous cells demonstrated a characteristic Ag-staining pattern. Innervation of the corneal endothelium was not observed. The avian cornea and its epithelium apparently become innervated over the same developmental period in which the major transition from corneal opacity to transparency is achieved.