Genome of the candidate phylum Aminicenantes bacterium from a deep subsurface thermal aquifer revealed its fermentative saccharolytic lifestyle
Genome of the candidate phylum Aminicenantes bacterium from a deep subsurface thermal aquifer revealed its fermentative saccharolytic lifestyle
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DOI：
10.1007/s00792-018-01073-5
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发表时间：
2019-03-01
期刊：
影响因子：
2.9
通讯作者：
Ravin, Nikolai V.
中科院分区：
文献类型：
--
作者：
Kadnikov, Vitaly V.;Mardanov, Andrey V.;Ravin, Nikolai V.
作为微生物群落的分子分析的结果，已经在各种陆地和海洋生态系统中鉴定出候选门OP8（氨基菌）的细菌。到目前为止，还没有一个代表性的Aminicenetrine已被分离在纯培养。我们从西西伯利亚2公里深的地下热含水层的宏基因组中组装了一个Aminicenetrium成员的近乎完整的基因组，并使用基因组数据分析了这种细菌的代谢途径及其生态作用。这种细菌，命名为BY38，被预测为杆状，它缺乏鞭毛机构，但编码抽搐运动。对BY38基因组的分析揭示了多种糖基水解酶，其能够利用碳水化合物，包括几丁质、纤维素、淀粉、甘露糖、半乳糖、果糖、岩藻糖、鼠李糖、麦芽糖和阿拉伯糖。对代谢途径的研究表明，氨基青霉菌BY38是一种厌氧有机营养菌，能够发酵碳水化合物和蛋白质底物，并利用亚硝酸盐进行厌氧呼吸。在深层地下含水层中，氨基乙酸可能作为埋藏有机质的破坏剂，产生氢和乙酸盐。基于系统发育和基因组分析，新的细菌，建议被归类为Escherichidatusacericenanssubacericheum。
Bacteria of candidate phylum OP8 (Aminicenantes) have been identified in various terrestrial and marine ecosystems as a result of molecular analysis of microbial communities. So far, none of the representatives of Aminicenantes have been isolated in a pure culture. We assembled the near-complete genome of a member of Aminicenantes from the metagenome of the 2-km-deep subsurface thermal aquifer in Western Siberia and used genomic data to analyze the metabolic pathways of this bacterium and its ecological role. This bacterium, designated BY38, was predicted to be rod shaped, it lacks flagellar machinery but twitching motility is encoded. Analysis of the BY38 genome revealed a variety of glycosyl hydrolases that can enable utilization of carbohydrates, including chitin, cellulose, starch, mannose, galactose, fructose, fucose, rhamnose, maltose and arabinose. The reconstructed central metabolic pathways suggested that Aminicenantes bacterium BY38 is an anaerobic organotroph capable of fermenting carbohydrates and proteinaceous substrates and performing anaerobic respiration with nitrite. In the deep subsurface aquifer Aminicenantes probably act as destructors of buried organic matter and produce hydrogen and acetate. Based on phylogenetic and genomic analyses, the novel bacterium is proposed to be classified as Candidatus Saccharicenans subterraneum.