Dissection of functional domains of adenovirus DNA polymerase by linker-insertion mutagenesis.
Dissection of functional domains of adenovirus DNA polymerase by linker-insertion mutagenesis.
复制标题
通过接头插入诱变对腺病毒 DNA 聚合酶的功能域进行解剖。
DOI：
10.1073/pnas.86.16.6116
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发表时间：
1989
影响因子：
11.1
通讯作者：
Horwitz,MS
中科院分区：
文献类型：
--
作者：
Chen,M;Horwitz,MS
将连接子插入突变引入克隆的腺病毒DNA聚合酶基因，并测量其对体外DNA起始和延伸的功能影响。聚合酶的基本区域似乎分散在整个分子中的补丁中，并且不限于与各种其他复制聚合酶共享的五个同源区域。因此，腺病毒DNA聚合酶可能含有活性位点，这些活性位点必须通过聚合酶分子间的远距离相互作用形成。
Linker-insertion mutations were introduced into the cloned adenovirus DNA polymerase gene and the functional effects on the initiation and elongation of DNA in vitro were measured. Essential regions of the polymerase appear to be scattered in patches across the entire molecule and are not limited to the five regions of homology shared with a variety of other replicating polymerases. Thus, the adenovirus DNA polymerase presumably contains active sites that must be formed by distant interactions across the polymerase molecule.