Cardiac microdialysis in isolated rat hearts: interstitial purine metabolites during ischemia.
Cardiac microdialysis in isolated rat hearts: interstitial purine metabolites during ischemia.
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离体大鼠心脏的心脏微透析：缺血期间的间质嘌呤代谢物。
DOI：
10.1152/ajpheart.1992.262.6.h1934
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
MentzerJr,RM
中科院分区：
文献类型：
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作者：
VanWylen,DG;Schmit,TJ;Lasley,RD;Gingell,RL;MentzerJr,RM
心脏微透析是最近发展起来的一种技术，它允许通过在心肌内植入和灌流中空的小透析纤维来采集心肌间质液体(ISF)。本文的目的是描述心脏微透析在离体灌流心脏中的初步研究。将自制的微透析探针植入大鼠离体心灌流心肌内，以0.5微升/分钟的Krebs-Henseleit缓冲液灌注。将流出的透析液测定腺苷、肌苷、次黄嘌呤、黄嘌呤和尿酸，作为这些嘌呤代谢物在心肌内水平的指标。所有代谢物在植入后开始升高，在前45分钟迅速下降，然后在接下来的90分钟内保持稳定。根据体外恢复百分率数据，外推基线透析液浓度以估计心肌内ISF(以微米为单位)：0.47腺苷、0.85肌苷、0.29次黄嘌呤、0.49黄嘌呤和8.6尿酸。在全球零血流缺血期间(37摄氏度)，所有嘌呤代谢物的透析液水平都升高，其中肌苷是主要化合物。用50微米红血球-9-(2-羟基-3-壬基)腺嘌呤(腺苷脱氨酶抑制剂)处理心脏，可显著促进ISF腺苷蓄积，并减弱肌苷、次黄嘌呤和黄嘌呤的蓄积。心脏微透析的简单性和多功能性表明，这项技术可能是使用该制剂进行的许多研究的有价值的辅助手段。
Cardiac microdialysis is a recently developed technique that allows intramyocardial interstitial fluid (ISF) to be sampled via the implantation and perfusion of a small, hollow dialysis fiber within the myocardium. The purpose of this paper is to describe initial studies using cardiac microdialysis in the isolated perfused heart. Microdialysis probes, constructed in the laboratory, were implanted in the left ventricular myocardium of isolated perfused rat hearts and perfused at 0.5 microliter/min with Krebs-Henseleit buffer. The effluent dialysate, assayed for adenosine, inosine, hypoxanthine, xanthine, and uric acid, was used as an index of intramyocardial levels of these purine metabolites. All metabolites were elevated initially after implantation, declined rapidly in the first 45 min, and were then stable for the next 90 min. Based on in vitro percent recovery data, baseline dialysate concentrations were extrapolated to yield estimates of intramyocardial ISF (in microM) 0.47 adenosine, 0.85 inosine, 0.29 hypoxanthine, 0.49 xanthine, and 8.6 uric acid. During global zero-flow ischemia (37 degrees C), dialysate levels of all purine metabolites were elevated, with inosine being the predominant compound. Pretreatment of the hearts with 50 microM erythro-9-(2-hydroxy-3-nonyl)adenine, an adenosine deaminase inhibitor, markedly enhanced ISF adenosine accumulation and attenuated the accumulation of inosine, hypoxanthine, and xanthine. The simplicity and versatility of cardiac microdialysis in the isolated perfused heart suggest that this technique may be a valuable adjunct to the many studies performed using this preparation.