Assembly of D1/D2 complexes of photosystem II: Binding of pigments and a network of auxiliary proteins.
Assembly of D1/D2 complexes of photosystem II: Binding of pigments and a network of auxiliary proteins.
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DOI：
10.1093/plphys/kiac045
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发表时间：
2022-06-01
期刊：
影响因子：
7.4
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中科院分区：
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光系统II （PSII）是一种多亚基的光驱动氧化还原酶，它利用从水中提取的电子驱动光合作用中的电子传递。为了研究PSII组装的初始步骤，我们使用在PSII生物发生早期捕获并表达亲和标记的PSII亚基的蓝藻细菌PCC 6803菌株分离出PSII反应中心组装（RCII）复合物及其前体D1和D2模块（D1mod和D2mod）。使用his标记的D2或flag标记的PsbI亚基分离的RCII制剂含有先前描述的RCIIa和RCII*复合物，其不同之处是Ycf39组装因子和高光诱导蛋白（Hlips）的存在，以及由RCIIa结合单体PSI组成的更大复合物。所有RCII复合物都含有PSII亚基D1、D2、PsbI、PsbE和PsbF以及rubredoxin A和Ycf48组装因子，但我们也检测到PsbN、Slr1470和Slr0575蛋白，这些蛋白都有植物同源物。RCII制剂还含有功能未知的禁止素/抑口素（Phbs）和FtsH蛋白酶亚基。RCII复合物在光诱导的初级电荷分离和结合叶绿素（Chls）、叶绿素、β -胡萝卜素和血红素中具有活性。分离得到的D1mod含有D1/PsbI/Ycf48和部分Ycf39和Phb3， D2mod含有D2/细胞色素b559，共纯化PsbY、Phb1、Phb3、FtsH2/FtsH3、CyanoP和Slr1470。由于稳定结合，Chl在D1mod中检测到，而在D2mod中检测不到，RCII的形成似乎对大多数Chl和两种叶绿素的稳定结合很重要。我们认为Chl可以通过单体光系统I或Ycf39/Hlips复合物传递到RCII。对分离组装复合物的分析为光系统II生物发生的早期阶段提供了新的见解。
Photosystem II (PSII) is the multi-subunit light-driven oxidoreductase that drives photosynthetic electron transport using electrons extracted from water. To investigate the initial steps of PSII assembly, we used strains of the cyanobacterium Synechocystis sp. PCC 6803 arrested at early stages of PSII biogenesis and expressing affinity-tagged PSII subunits to isolate PSII reaction center assembly (RCII) complexes and their precursor D1 and D2 modules (D1mod and D2mod). RCII preparations isolated using either a His-tagged D2 or a FLAG-tagged PsbI subunit contained the previously described RCIIa and RCII* complexes that differ with respect to the presence of the Ycf39 assembly factor and high light-inducible proteins (Hlips) and a larger complex consisting of RCIIa bound to monomeric PSI. All RCII complexes contained the PSII subunits D1, D2, PsbI, PsbE, and PsbF and the assembly factors rubredoxin A and Ycf48, but we also detected PsbN, Slr1470, and the Slr0575 proteins, which all have plant homologs. The RCII preparations also contained prohibitins/stomatins (Phbs) of unknown function and FtsH protease subunits. RCII complexes were active in light-induced primary charge separation and bound chlorophylls (Chls), pheophytins, beta-carotenes, and heme. The isolated D1mod consisted of D1/PsbI/Ycf48 with some Ycf39 and Phb3, while D2mod contained D2/cytochrome b559 with co-purifying PsbY, Phb1, Phb3, FtsH2/FtsH3, CyanoP, and Slr1470. As stably bound, Chl was detected in D1mod but not D2mod, formation of RCII appears to be important for stable binding of most of the Chls and both pheophytins. We suggest that Chl can be delivered to RCII from either monomeric Photosystem I or Ycf39/Hlips complexes. Analysis of isolated assembly complexes provides new insights into the early stages of photosystem II biogenesis.
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CP43无光系统II的亚基组成，Synechocystis sp。 PCC 6803：对光系统II的组装和修复的影响。
DOI：
10.1098/rstb.2012.0066
发表时间：
2012-12-19
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Boehm M;Yu J;Reisinger V;Beckova M;Eichacker LA;Schlodder E;Komenda J;Nixon PJ
通讯作者：
Nixon PJ
影响因子：
7.4
作者：
Dobakova, Marika;Sobotka, Roman;Komenda, Josef
通讯作者：
Komenda, Josef
影响因子：
10.7
作者：
Janouskovec, Jan;Sobotka, Roman;Keeling, Patrick J.
通讯作者：
Keeling, Patrick J.
影响因子：
4.9
作者：
Knoppova, Jana;Yu, Jianfeng;Komenda, Josef
通讯作者：
Komenda, Josef
影响因子：
16.8
作者：
Guskov, Albert;Kern, Jan;Saenger, Wolfram
通讯作者：
Saenger, Wolfram