The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum
The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum
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DOI：
10.1111/j.1364-3703.2010.00612.x
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发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Di Pietro, Antonio
中科院分区：
文献类型：
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作者：
Rispail, Nicolas;Di Pietro, Antonio
P>真菌组氨酸激酶 (HK) 与不同的过程有关，例如渗透压反应、菌丝发育、对杀菌剂的敏感性和毒力。已知 HK III 类成员通过 HOG 丝裂原激活蛋白激酶 (MAPK) 发出信号，但尚未探索与其他 MAPK 的可能相互作用。在这项研究中，我们鉴定了 fhk1，它编码来自土传维管束枯萎病菌尖孢镰刀菌的假定 III 类 HK。 fhk1 失活导致对苯基吡咯和二甲酰亚胺杀菌剂的抗性，以及对高渗应激和甲萘醌诱导的氧化应激的敏感性增加。 Delta fhk1 突变体的渗透敏感性与菌落形态的显着且先前未报道的变化有关。 Delta fhk1 菌株对番茄植株的毒力显着降低。 Fhk1 和 Fmk1 MAPK 级联之间的上位分析表明，Fhk1 不在 Fmk1 上游发挥作用，但这两条途径可能相互作用以控制对甲萘醌诱导的氧化应激的反应。
P>Fungal histidine kinases (HKs) have been implicated in different processes, such as the osmostress response, hyphal development, sensitivity to fungicides and virulence. Members of HK class III are known to signal through the HOG mitogen-activated protein kinase (MAPK), but possible interactions with other MAPKs have not been explored. In this study, we have characterized fhk1, encoding a putative class III HK from the soil-borne vascular wilt pathogen Fusarium oxysporum. Inactivation of fhk1 resulted in resistance to phenylpyrrole and dicarboximide fungicides, as well as increased sensitivity to hyperosmotic stress and menadione-induced oxidative stress. The osmosensitivity of Delta fhk1 mutants was associated with a striking and previously unreported change in colony morphology. The Delta fhk1 strains showed a significant decrease in virulence on tomato plants. Epistatic analysis between Fhk1 and the Fmk1 MAPK cascade indicated that Fhk1 does not function upstream of Fmk1, but that the two pathways may interact to control the response to menadione-induced oxidative stress.