Dynamic cell adhesion and viscoelastic signatures distinguish normal from malignant human mammary cells using quartz crystal microbalance.
Dynamic cell adhesion and viscoelastic signatures distinguish normal from malignant human mammary cells using quartz crystal microbalance.
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DOI：
10.1016/j.ab.2011.10.052
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发表时间：
2012-02
影响因子：
2.9
通讯作者：
Tiean Zhou;K. Marx;Abiche H. Dewilde;D. McIntosh;S. Braunhut
中科院分区：
文献类型：
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作者：
Tiean Zhou;K. Marx;Abiche H. Dewilde;D. McIntosh;S. Braunhut
在正常细胞转变为能够形成癌性生长的细胞的过程中，细胞形态、细胞骨架和焦点接触发生显着变化。这些变化应该能够通过实时监测动态细胞粘附过程和细胞的粘弹性特性来表征。在这里，我们描述了使用石英晶体微天平（QCM）来区分人类正常（HMEC）与恶性（MCF-7）乳腺上皮细胞的动态细胞粘附特征。与 HMEC 相比，MCF-7 细胞的 QCM 响应（频率 [Δf] 和运动阻力 ΔR 的变化）显着降低，反映了通过光学显微镜观察到的癌细胞的形态特征。我们分析了最初的 2 小时细胞粘附动力学，表明 HMEC 具有细胞间协同作用，而 MCF-7 细胞则没有或微弱的细胞间相互作用。我们提出，ΔR/Δf比率的变化，即细胞粘弹性指数（CVI），反映了活细胞细胞骨架结构和动态粘弹性特性的建立。当细胞建立其细胞骨架结构时，细胞与表面相互作用开始时，CVI 显着降低。在细胞粘附过程中，MCF-7 细胞始终更柔软，与 HMEC 相比，其 CVI 小 2.5 倍。
During transformation of a normal cell to a cell capable of forming a cancerous growth, cellular morphology, the cytoskeleton, and focal contacts undergo significant changes. These changes should be capable of being characterized via real-time monitoring of the dynamic cell adhesion process and viscoelastic properties of cells. Here, we describe use of the quartz crystal microbalance (QCM) to distinguish the dynamic cell adhesion signatures of human normal (HMEC) versus malignant (MCF-7) mammary epithelial cells. The significantly reduced QCM responses (changes in frequency [Δf] and motional resistance ΔR) of MCF-7 cells compared with those of HMECs mirror the cancer cells’ morphological features as observed via optical microscope. We analyzed the initial 2-h cell adhesion kinetics, suggesting cell–cell cooperativity for HMECs and no or weak cell–cell interactions for MCF-7 cells. We propose that changes of the ΔR/Δf ratio, which we term the cell viscoelastic index (CVI), reflect the establishment of cytoskeleton structure and dynamic viscoelastic properties of living cells. The CVI decreases significantly on initiation of cell to surface interactions as cells establish their cytoskeletal structures. During the cell adhesion process, MCF-7 cells were consistently softer, exhibiting up to a 2.5-fold smaller CVI when compared with HMECs.