Steroidogenic effect of 17 beta-estradiol in the rabbit: stimulation of progesterone synthesis in prematurely regressing corpora lutea.
Steroidogenic effect of 17 beta-estradiol in the rabbit: stimulation of progesterone synthesis in prematurely regressing corpora lutea.
复制标题
17β-雌二醇对兔子的类固醇生成作用：刺激黄体过早退化的黄体酮合成。
作者：
E. Bender;J. Miller;R. Possley;P. Keyes
研究了雌二醇可能对兔黄体具有类固醇生成作用的可能性。首先迅速撤除雌二醇以使血清孕酮部分下降，然后补充雌二醇以确定是否能刺激黄体孕酮的合成。在假孕的第1天（无菌交配后的第1天），皮下植入填充雌二醇的硅橡胶囊，它可使血清雌二醇迅速升高5 - 6倍，并以恒定速率释放雌二醇。雌二醇植入物一直放置到第10天，然后取出。在第11天，要么重新植入，要么进行假植入。第10天血清孕酮为14纳克/毫升，在取出雌二醇植入物后，24小时内下降到3.9纳克/毫升。在第11天重新植入雌二醇后，血清孕酮在12小时内升高到6.5纳克/毫升，并在第15 - 17天继续上升到平均值为12 - 13纳克/毫升。在未重新植入雌二醇的动物中，血清孕酮继续……
The possibility that estradiol might have a steroidogenic effect on the rabbit corpus luteum was investigated, first by rapid withdrawal of estradiol to allow a partial decline in serum progesterone, followed by replacement of estradiol to determine whether luteal progesterone synthesis could be stimulated. Estradiolfilled Silastic capsules, which cause rapid, 5- to 6-fold elevations in serum estradiol and which release estradiol at constant rates, were implanted sc on day 1 (the day after sterile mating) of pseudopregnancy. The estradiol implant was left in place until day 10, when it was removed. On day 11, the implant was either replaced or sham replaced. Serum progesterone on day 10 was 14 ng/ml and, after removal of estradiol implants, decreased to 3.9 ng/ml within 24 h. After replacement of estradiol on day 11, serum progesterone increased to 6.5 ng/ml within 12 h and continued to rise to mean values of 12-13 ng/ml on days 15-17. In animals without estradiol replacement, serum progesterone continued...