Analysis of Proprioceptive Sensory Innervation of the Mouse Soleus: A Whole-Mount Muscle Approach.
Analysis of Proprioceptive Sensory Innervation of the Mouse Soleus: A Whole-Mount Muscle Approach.
复制标题
小鼠比目鱼的本体感受感官神经的分析：一种整体肌肉方法。
DOI：
10.1371/journal.pone.0170751
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ladle DR
中科院分区：
文献类型：
--
作者：
Sonner MJ;Walters MC;Ladle DR
肌肉本体感觉传入神经通过骨骼肌内的专门机械感受器提供对成功执行运动任务至关重要的反馈：由 Ia 组和 II 组传入神经提供的肌梭，以及由 Ib 组传入神经提供的高尔基腱器官。这些本体感受器及其相关传入神经的形态已在猫比目鱼肌中进行了广泛研究，在大鼠中也进行了较小程度的研究。然而，对小鼠比目鱼肌本体感觉神经支配的定量分析相对有限。本研究采用基因编码荧光报告系统，使用高放大倍数共聚焦显微镜来标记和分析完整小鼠比目鱼肌内的肌梭、高尔基腱器官和本体感觉神经元亚群。肌梭和高尔基腱器官的本体感觉受体总数分别为 11.3 ± 0.4 和 5.2 ± 0.2，并且这些受体计数独立变化（n = 27 块肌肉）。与大鼠的研究结果类似，所分析的肌梭最常由两个本体感觉传入神经提供，并且在大多数情况下，使用既定的形态学标准将两者都归类为初级末梢。当纺锤体相关传入总数达到三个或更多时，最常观察到次要结局。初级和次级传入轴突的平均直径存在显着差异，但分布重叠程度比之前在猫和大鼠研究中观察到的要多。
Muscle proprioceptive afferents provide feedback critical for successful execution of motor tasks via specialized mechanoreceptors housed within skeletal muscles: muscle spindles, supplied by group Ia and group II afferents, and Golgi tendon organs, supplied by group Ib afferents. The morphology of these proprioceptors and their associated afferents has been studied extensively in the cat soleus, and to a lesser degree, in the rat; however, quantitative analyses of proprioceptive innervation in the mouse soleus are comparatively limited. The present study employed genetically-encoded fluorescent reporting systems to label and analyze muscle spindles, Golgi tendon organs, and the proprioceptive sensory neuron subpopulations supplying them within the intact mouse soleus muscle using high magnification confocal microscopy. Total proprioceptive receptors numbered 11.3 ± 0.4 and 5.2 ± 0.2 for muscle spindles and Golgi tendon organs, respectively, and these receptor counts varied independently (n = 27 muscles). Analogous to findings in the rat, muscle spindles analyzed were most frequently supplied by two proprioceptive afferents, and in the majority of instances, both were classified as primary endings using established morphological criteria. Secondary endings were most frequently observed when spindle associated afferents totaled three or more. The mean diameter of primary and secondary afferent axons differed significantly, but the distributions overlap more than previously observed in cat and rat studies.