Lumen morphogenesis in C-elegans requires the membrane-cytoskeleton linker erm-1
Lumen morphogenesis in C-elegans requires the membrane-cytoskeleton linker erm-1
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DOI：
10.1016/j.devcel.2004.05.018
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发表时间：
2004-06-01
影响因子：
11.8
通讯作者：
Fleming, JT
中科院分区：
文献类型：
--
作者：
Göbel, V;Barrett, PL;Fleming, JT
上皮管是复杂器官的基本组成部分，但其结构要求尚不清楚。在这里，我们证明erm-1是细胞骨架-膜连接子ERM家族的独特秀丽隐杆线虫直系同源物，在管腔形态发生中发挥重要作用。 ERM-1定位于那些缺乏角质层稳定作用的管状器官上皮细胞的管腔膜。 RNA 干扰 (RNAi)、种系缺失和 erm-1 过度表达会导致这些上皮细胞出现囊性管腔表型。共聚焦和超微结构分析表明，erm-1 直接在顶膜形态发生中发挥作用，而不是像之前针对 ERM 提出的那样，在上皮极性和连接组装中发挥作用。我们还表明，act-5/细胞质肌动蛋白和 sma-1/P-H-血影蛋白是管腔形成所必需的，并且与 erm-1 功能上相互作用。我们的研究结果表明，不同器官管腔的结构存在共同的结构限制。
Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/ P-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.