The search for origins of DNA replication
The search for origins of DNA replication
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DOI：
10.1006/meth.1997.0521
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发表时间：
1997-11-01
期刊：
影响因子：
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通讯作者：
DePamphilis, ML
中科院分区：
文献类型：
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作者：
DePamphilis, ML
过去十年，有关真核细胞 DNA 复制性质的新信息激增。这些信息大部分源自用于识别和表征病毒、质粒和细胞基因组中 DNA 复制起源的新方法的出现。这些方法可以非常精确地绘制复制开始的位点。此外，它们还提供了起始活性测定，可用于识别导致染色体 DNA 特定位点复制前复合物形成和激活的事件序列。我简要总结了当前对真核复制起源的看法以及用于识别和表征它们的方法。随后的文章将详细描述那些对未来应用有前景的选定方法。 (C) 1997 年学术出版社。
The past decade has witnessed an explosion of new information about the nature of DNA replication in eukaryotic cells. Much of this information has resulted from the advent of novel methods for identifying and characterizing origins of DNA replication in the genomes of viruses, plasmids, and cells. These methods can map with remarkable precision sites where replication begins. In addition, they provide assays for origin activity that can be used to identify the sequence of events leading to the formation and activation of prereplication complexes at specific sites in chromosomal DNA. I summarize briefly the current view of eukaryotic replication origins and the methods that have been used to identify and characterize them. Selected methods that show promise for future applications are then described in detail in subsequent articles. (C) 1997 Academic Press.