Molecular typing of bacteria directly from cerebrospinal fluid
Molecular typing of bacteria directly from cerebrospinal fluid
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DOI：
10.1007/s100960000321
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发表时间：
2000-08-01
影响因子：
4.5
通讯作者：
Spratt, BG
中科院分区：
文献类型：
--
作者：
Enright, MC;Knox, K;Spratt, BG
以肺炎链球菌为例，研究了多位点序列分型（MLST）对直接从脑脊液（CSF）分离的肺炎链球菌的鉴定能力。采用巢式多重聚合酶链反应方法对30例疑似细菌性脑膜炎病例的脑脊液样本进行扩增，该方法可扩增用于肺炎球菌MLST的7个管家基因片段。从培养肺炎链球菌的所有14个样本中扩增出这些片段，在直接测序后，从10个样本中获得的等位基因谱与先前与侵袭性肺炎球菌疾病相关的克隆相对应。MLST还能预测血清型和青霉素敏感性。
Using Streptococcus pneumoniae as an example, the ability of multilocus sequence typing (MLST) to characterise isolates directly from cerebrospinal fluid (CSF) was investigated. A nested multiplex polymerase chain reaction method that amplifies the seven housekeeping gene fragments used for pneumococcal MLST was applied to 30 CSF samples from suspected cases of bacterial meningitis. The fragments were amplified from all 14 samples from which Streptococcus pneumoniae was cultured, and, after direct sequencing, the allelic profiles obtained from ten of the samples corresponded to those of clones previously associated with invasive pneumococcal disease. MLST could also predict the penicillin susceptibility and serotype of the CSF isolates.