Induction of mesenchymal progenitor cells with chondrogenic property from mouse-induced pluripotent stem cells.
Induction of mesenchymal progenitor cells with chondrogenic property from mouse-induced pluripotent stem cells.
复制标题
DOI：
10.1089/cell.2009.0086
复制
发表时间：
2010-06
影响因子：
1.6
通讯作者：
Takeshi Teramura;Yuta Onodera;Toshihiro Mihara;Y. Hosoi;C. Hamanishi;Kanji Fukuda
中科院分区：
文献类型：
--
作者：
Takeshi Teramura;Yuta Onodera;Toshihiro Mihara;Y. Hosoi;C. Hamanishi;Kanji Fukuda
尽管最近细胞工程取得了进展，但软骨的治疗和修复仍然具有挑战性。尽管使用间充质干细胞（MSC）的干细胞移植治疗被认为是一种重要的策略，但自体细胞增殖能力有限的主要问题尚未解决。最近，诱导多能干（iPS）细胞系被建议作为治疗各种人类疾病的替代方法，因为它们具有无限增殖的潜力，同时具有形成所有类型细胞的能力。然而，诱导谱系限制分化的方法尚未得到充分检验或建立。在这里，我们提出了一种从小鼠 iPS 细胞中诱导具有软骨形成特性的间充质祖细胞的简单方法。我们研究中产生的MSC样细胞表达一些MSC标志物，并且还可以分化为成骨细胞和脂肪细胞。本研究证明了 iPS 细胞作为治疗关节疾病的替代候选者的特性，并提出了一种从 iPS 细胞制备软骨细胞的有效方法。
Despite recent cell-engineering advances, treatment and repair of cartilage remains challenging. Although stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a prominent strategy, the major problem of limited proliferative capacity of autologous cells has been unsolved. Recently, an induced pluripotent stem (iPS) cell line was suggested as an alternative way to cure various human diseases due to their potential proliferating infinitely while possessing the capacity to form all types of cells. However, the method to induce lineage-restricted differentiation has not been well examined or established. Here, we suggest a simple method to induce mesenchymal progenitors possessing chondrogenic property from mouse iPS cells. The MSC-like cells produced in our study expressed some MSC markers, and could also differentiate to osteoblast and adipocyte. The present study demonstrates the property of iPS cells as an alternative candidate for treatment of articular disorders, and suggests an effective approach for preparing chondrocyte from iPS cells.