Purification of immunosuppressive factor from Capnocytophaga ochracea
Purification of immunosuppressive factor from Capnocytophaga ochracea
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DOI：
10.1099/00222615-47-12-1087
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发表时间：
1998-12-01
影响因子：
3
通讯作者：
Kurita-Ochiai, T
中科院分区：
文献类型：
--
作者：
Ochiai, K;Senpuku, H;Kurita-Ochiai, T
头细胞吞噬菌是口腔细菌的一个属，与多种疾病的发病有关，包括心内膜炎、败血症和口腔疾病，如脓肿和牙周病。这项研究检测了Capnocytophaga菌株的声波提取物(SE)改变淋巴细胞功能的能力。受试者吞噬头细胞的SE能剂量依赖性地抑制脾细胞对有丝分裂原的反应。抑制因子(SF)经超凝胶-AcA34柱层析、高压液体DEAE离子交换层析和羟基磷灰石柱层析分离纯化，经SDS-PAGE显示为一条约14 kDa的单一条带。纯化的SF兔抗血清可抑制SE诱导的免疫抑制，恢复淋巴细胞增殖。
Capnocytophaga, one of the genera of oral bacteria, has been implicated in the pathogenesis of several diseases, including endocarditis, septicaemia and disorders of the oral cavity such as abscesses and periodontal disease. This study examined sonic extracts (SE) of Capnocytophaga strains for their ability to alter lymphocyte function. The SE of tested Capnocytophaga caused dose-dependent suppression of spleen cells in response to mitogen. This suppressive effect was heat-labile and sensitive to the proteolytic enzyme pronase E. The suppressive factor (SF) was purified from SE of C. ochrasea by a combination of ultrogel-AcA34, high-pressure liquid DEAE ion-exchange chromatography and hydroxyapatite columns, which revealed a single band of 14 kDa by SDS-PAGE. Rabbit anti-serum against the purified SF inhibited the immunosuppression induced by SE of C. ochracea with the recovery of lymphocyte proliferation.