Modulation of C:N:P stoichiometry is involved in the effectiveness of a PGPR and AM fungus in increasing salt stress tolerance of Sulla carnosa Tunisian provenances
Modulation of C:N:P stoichiometry is involved in the effectiveness of a PGPR and AM fungus in increasing salt stress tolerance of Sulla carnosa Tunisian provenances
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DOI：
10.1016/j.apsoil.2019.06.014
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发表时间：
2019-11-01
影响因子：
4.8
通讯作者：
Azcon, Rosario
中科院分区：
文献类型：
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作者：
Hidri, Rabaa;Metoui-Ben Mahmoud, Ouissal;Azcon, Rosario
肉质岩黄芪（Hedysarum carnosum Desf.）是一种重要的豆类作物，在受盐影响的地区作为动物饲料种植。在这里，我们评估的影响，植物生长促进细菌（PGPR）（枯草芽孢杆菌）与或没有菌根真菌（AMF）Rhizophagus intraradices对四个S。carnosa突尼斯种源（Sidi khlif，Thelja，Kalbia和Kerker）生长在温室中的盐水和非盐水条件下。植物生长，矿质营养，C：N：P化学计量在植物芽，和土壤酶活性被认为是。微生物对植物（根和芽）生长的影响范围从微不足道的Kerker高度积极的种源Sidi khlif和Kalbia。在后者种源中，双接种对生长的积极影响比单接种更明显。与对照相比，盐胁迫下植物生长显著下降，但Sidi khlif种源受到的影响最严重。特别是在Kalbia，地上部矿质元素（N，P，K，Mg，Cu和Fe）含量在盐胁迫下显著增加。与盐胁迫和未接种的植物相比，根内。这表明接种R.在苗圃建立过程中的根内，有助于缓解盐胁迫，保持有利的营养配置文件在Kalbia种源。用B.枯草芽孢杆菌表现出不同的C：N：P化学计量相比，盐处理和未接种的植物，表明B。枯草芽孢杆菌可以通过营养分配模式提高植物对盐胁迫的耐受性。B的效率枯草芽孢杆菌在Kalbia和Thelja中的优势是由于该菌株在胁迫条件下的高IAA产量。与未接种的对照相比，施用PGPR和AMF通过提高土壤酶活性（如脲酶、碱性磷酸酶、β-葡萄糖苷酶和脱氢酶）来改善土壤质量。因此，我们认为接种微生物是必要的。在盐和非盐条件下，肉苁蓉种源达到其最佳营养。因此，使用适当的微生物接种物和种源可能是一个关键问题，成功的生态稳定性，这地中海豆科植物，从而提高其适用于盐碱地的补救。
Sulla carnosa (Hedysarum carnosum Desf.) is an important legume grown as animal feed on salt-affected areas. Here, we assess the influence of plant-growth-promoting bacteria (PGPR) (Bacillus subtilis) with or without the mycorrhizal fungus (AMF) Rhizophagus intraradices on four S. carnosa Tunisian provenances (Sidi khlif, Thelja, Kalbia and Kerker) grown in the greenhouse under saline and non-saline conditions. Plant growth, mineral nutrition, C:N:P stoichiometry in plant shoots, and soil enzymatic activities were considered. Microbial effectiveness on plant (roots and shoots) growth ranged from insignificant in Kerker to highly positive in the provenances Sidi khlif and Kalbia. In the latter provenance, the positive effect on growth was more pronounced under dual than single inoculation. Plant growth declined significantly under salt stress compared to the control, but Sidi khlif provenance was the most severely impacted. Especially in Kalbia, shoot mineral (N, P, K, Mg, Cu, and Fe) concentrations increased significantly in salt-stressed plants inoculated with R. intraradices as compared to the salt-stressed and non-inoculated plants. This suggests that inoculation with R. intraradices during nursery establishment contributes to salt stress alleviation by maintaining a favourable nutrient profile in Kalbia provenance. Shoots of Sidi khlif plants inoculated with B. subtilis demonstrated distinct C:N:P stoichiometry as compared to salt-treated and non-inoculated plants, indicating that B. subtilis could increase plant tolerance to salt stress through nutrient allocation patterns. The efficiency of B. subtilis in Kalbia and Thelja was due to the high IAA production by this strain under stress conditions. Application of PGPR and AMF improved soil quality by increasing the activities of soil enzyme such as urease, alkaline phosphatase, beta-glucosidase and dehydrogenase compared to their respective non-inoculated controls. From this study, we conclude that microbial inoculation was necessary for S. carnosa provenances to reach their optimal nutrition under saline and non-saline conditions. Thus, the use of adequate microbial inocula and provenance could be a critical issue for the successful ecological stability of this Mediterranean legume, thereby improving its suitability for the remediation of saline lands.