INTERFERON-ALPHA AND INTERFERON-BETA DOWN-REGULATE THE EXPRESSION OF BASIC FIBROBLAST GROWTH-FACTOR IN HUMAN CARCINOMAS
INTERFERON-ALPHA AND INTERFERON-BETA DOWN-REGULATE THE EXPRESSION OF BASIC FIBROBLAST GROWTH-FACTOR IN HUMAN CARCINOMAS
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DOI：
10.1073/pnas.92.10.4562
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
FIDLER, IJ
中科院分区：
文献类型：
--
作者：
SINGH, RK;GUTMAN, M;FIDLER, IJ
We investigated the influence of interferons alpha, beta, and gamma (IFN-alpha, -beta, and -gamma) on the production of basic fibroblast growth factor (bFGF) by human renal carcinoma cells. The human renal carcinoma cell metastatic line SN12PM6 was established in culture from a lung metastasis and SN12PM6-resistant cells were selected in vitro for resistance to the antiproliferative effects of IFN-alpha or IFN-beta. IFN-alpha and IFN-beta, but not IFN-gamma, down-regulated the expression of bFGF at the mRNA and protein levels by a mechanism independent of their antiproliferative effects. Downregulation of bFGF required a long exposure (> 4 days) of cells to low concentrations (>10 units/ml) of IFN-alpha or IFN-beta. The withdrawal of IFN-alpha or IFN-beta from the medium permitted SN12PM6-resistant cells to resume production of bFGF. The incubation of human bladder, prostate, colon, and breast carcinoma cells with noncytostatic concentrations of IFN-alpha or IFN-beta also produced down-regulation of bFGF production.
