Identification and distinct regulation of yeast TATA box-containing genes
Identification and distinct regulation of yeast TATA box-containing genes
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DOI：
10.1016/s0092-8674(04)00205-3
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发表时间：
2004-03-05
期刊：
影响因子：
64.5
通讯作者：
Pugh, BF
中科院分区：
文献类型：
--
作者：
Basehoar, AD;Zanton, SJ;Pugh, BF
尽管是第一个真核转录调控元件之一，天然TATA盒的序列及其意义仍然难以捉摸。应用与TATA框相关的标准，我们查询了几个酵母基因组，并达成了共识TATA（A/T）A（A/T）（A/G）。大约20%的酵母基因含有TATA盒。引人注目的是，含有TATA盒的基因与应激反应相关，受到高度调节，并且与TATA较少的启动子相比，优先利用佐贺而不是TFIID。酵母中的转录调控似乎是机械双极的，可能反映了需要平衡诱导应激相关反应与组成性管家功能。
Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.