Molecular mechanism of phosphorous signaling inducing anthocyanin accumulation in Arabidopsis
Molecular mechanism of phosphorous signaling inducing anthocyanin accumulation in Arabidopsis
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磷信号诱导拟南芥花青素积累的分子机制
DOI：
10.1016/j.plaphy.2023.01.029
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发表时间：
2023-01-25
影响因子：
6.5
通讯作者：
Hu, Yanru
中科院分区：
文献类型：
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作者：
Li, Huiqiong;He, Kunrong;Hu, Yanru
花色苷是一种次生代谢途径产生的黄酮类化合物，在多种生物过程中发挥着重要作用。磷是植物生长发育所必需的大量元素，磷饥饿通常导致花色素苷的积累。然而，缺磷促进花色苷生物合成的分子机制尚未得到很好的表征。在这里，我们提供了证据表明，P信号核心蛋白磷酸饥饿反应1（PHR 1）与参与花青素生物合成的转录因子（TF），包括花青素色素的产生1（PAP 1/MYB 75），MYB结构域蛋白113（MYB 113）和透明种皮8（TT 8）物理相关。PHR 1及其同源物在缺磷条件下正调控拟南芥幼苗花青素苷积累。破坏PHR 1的同时呈现幼苗对限制P的低敏感性，而PHR 1的过表达增强了P缺乏诱导的花青素苷积累。遗传分析表明，35 S：PHR 12 HA-5幼苗部分恢复了myb-RNAi和tt 8突变体的缺磷不敏感表型。综上所述，我们的研究表明PHR 1和MBW复合物形成的蛋白复合物直接介导了缺磷诱导的花色素苷积累过程，为理解缺磷信号如何依赖内源花色素苷合成途径促进拟南芥花色素苷积累提供了新的机制。
Anthocyanins, flavonoid compounds derived from secondary metabolic pathways, play important roles in various biological processes. Phosphorus (P) is an essential macroelement for plant growth and development, and P-starvation usually results in anthocyanin accumulation. However, the molecular mechanism of P deficiency promotes anthocyanin biosynthesis has not been well characterized. Here, we provided evidence that the P signaling core protein PHOSPHATE STARVATION RESPONSE1 (PHR1) is physically associate with transcription factors (TFs) involved in anthocyanidin biosynthesis, including PRODUCTION OF ANTHOCYANIN PIGMENTS1 (PAP1/MYB75), MYB DOMAIN PROTEIN 113 (MYB113) and TRANSPARENT TESTA 8 (TT8). PHR1 and its homologies positively regulated anthocyanin accumulation in Arabidopsis seedlings under P-deficient conditions. Disruption of PHR1 simultaneously rendered seedlings hyposensitive to limiting P, whereas the overexpression of PHR1 enhanced P- deficiency-induced anthocyanin accumulation. Genetic analysis demonstrated that 35S:PHR12HA-5 seedlings partially recovers the P deficiency insensitive phenotype of myb-RNAi and tt8 mutants. In summary, our study indicated that protein complexes formed by PHR1 and MBW complex directly mediate the process of P-deficiency-induced anthocyanin accumulation, providing a new mechanistic understanding of how P-deficient signaling depends on the endogenous anthocyanin synthesis pathway to promote anthocyanin accumulation in Arabidopsis.