The oxidative metabolism of thioglycollate-elicited mouse peritoneal macrophages: the relationship between oxygen, superoxide and hydrogen peroxide and the effect of monolayer formation.
The oxidative metabolism of thioglycollate-elicited mouse peritoneal macrophages: the relationship between oxygen, superoxide and hydrogen peroxide and the effect of monolayer formation.
复制标题
巯基乙酸引发的小鼠腹腔巨噬细胞的氧化代谢：氧、超氧化物和过氧化氢之间的关系以及单层形成的影响。
作者：
M. Cohen;J. Ryan;R. Root
检查了从未处理小鼠（驻留细胞）和腹腔注射巯基乙酸盐肉汤（巯基乙酸盐细胞）的小鼠中收获的腹膜巨噬细胞的氧和葡萄糖代谢。巯基乙酸盐细胞消耗约3倍的O2在休息和吞噬过程中的居民细胞，但氧还原产物（超氧化物和过氧化氢）可以回收，尽管触发吞噬或暴露于PMA的只有最小的量。通过观察葡萄糖氧化和NBT染料还原的主要途径，获得了巯基乙酸盐细胞形成氧还原产物（如O2-）的间接证据。当巯基乙酸盐细胞被允许粘附到玻璃表面O2-和H2 O2很容易恢复在细胞外介质中的20倍以上的细胞悬浮液暴露于PMA。这项研究表明，巯基乙酸盐引起的巨噬细胞有一个有力的氧化代谢，但恢复，也许利用，形成的O2还原产物将取决于孵育的条件。这些事件对于体外巨噬细胞“活化”参数的研究以及这些细胞在体内的功能都可能是重要的。
The oxygen and glucose metabolism of peritoneal macrophages harvested from untreated mice (resident cells) and mice given an i.p. injection of thioglycollate broth (thioglycollate cells) were examined. Thioglycollate cells consumed approximately 3 times as much O2 at rest and during phagocytosis as resident cells, but oxygen reduction products (superoxide and hydrogen peroxide) could be recovered in only minimal amounts despite triggering by phagocytosis or exposure to PMA. Indirect evidence for the formation of oxygen reduction products such as O2- by thioglycollate cells was obtained by observation of the major pathways for glucose oxidation and NBT dye reduction. When thioglycollate cells were allowed to adhere to a glass surface O2- and H2O2 were easily recovered in the extracellular medium with a 20-fold increase above cells in suspension exposed to PMA. This study suggests that thioglycollate-elicited macrophages have a vigorous oxidative metabolism but that recovery, and perhaps utilization, of O2 reduction products formed will depend on the conditions of incubation. These events may be significant both for the study of parameters of macrophage "activation" in vitro as well as the function of these cells in vivo.