Antioxidant properties of butein isolated from Dalbergia odorifera
Antioxidant properties of butein isolated from Dalbergia odorifera
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DOI：
10.1016/s0005-2760(98)00043-5
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发表时间：
1998-06-15
期刊：
影响因子：
--
通讯作者：
Teng, CM
中科院分区：
文献类型：
--
作者：
Cheng, ZJ;Kuo, SC;Teng, CM
研究了从降香黄檀中分离得到的蛋白的抗氧化性能。Butein抑制铁诱导脂质过氧化反应在老鼠大脑匀浆浓度的方式IC50, 3.3 + / - 0.4μm的作用是α-生育酚在减少稳定自由基diphenyl-2-picrylhydrazyl IC0.200 (DPPH), 9 2 + / - 1.8μm .它也抑制黄嘌呤氧化酶的活性IC50, 5.9 + / - 0.3μm .此外,Butein回收过氧化氢自由基来源于2,2-azobis (2-amidinopropane)盐酸盐(AAPH)在水相,而不是2,2-偶氮（2,4-二甲基戊腈）（AMVN）在己烷中的反应。此外，通过共轭二烯和硫代巴比妥酸反应物质（TBARS）的形成，丁蛋白抑制了铜催化的人低密度脂蛋白（LDL）氧化，并以浓度依赖的方式抑制了电泳迁移率。光谱分析表明，丁蛋白是铁离子和铜离子的螯合剂。研究表明，卵磷脂具有清除自由基和金属离子螯合作用，是一种有效的抗脂质和低密度脂蛋白过氧化的抗氧化剂。(C) 1998爱思唯尔科学有限公司
The antioxidant properties of butein, isolated from Dalbergia odorifera T. Chen, were investigated in this study. Butein inhibited iron-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC50, 3.3 +/- 0.4 mu M. It was as potent as alpha-tocopherol in reducing the stable free radical diphenyl-2-picrylhydrazyl (DPPH) with an IC0.200, 9 2 +/- 1.8 mu M. It also inhibited the activity of xanthine oxidase with an IC50, 5.9 +/- 0.3 mu M. Besides, butein scavenged the peroxyl radical derived from 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) in aqueous phase, but not that from 2,2-azobis(2,4-dimethylvaleronitrile) (AMVN) in hexane. Furthermore, butein inhibited copper-catalyzed oxidation of human low-density lipoprotein (LDL), as measured by conjugated dienes and thiobarbituric acid-reactive substance (TBARS) formations, and electrophoretic mobility in a concentration-dependent manner. Spectral analysis revealed that butein was a chelator of ferrous and copper ions. It is proposed that butein serves as a powerful antioxidant against lipid and LDL peroxidation by its versatile free radical scavenging actions and metal ion chelation. (C) 1998 Elsevier Science B.V.