Quantitative PCR Method To Measure the Fungal Endophyte in Locoweeds
Quantitative PCR Method To Measure the Fungal Endophyte in Locoweeds
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DOI：
10.1021/jf901028z
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发表时间：
2009-07-22
影响因子：
6.1
通讯作者：
Green, Benedict T.
中科院分区：
文献类型：
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作者：
Cook, Daniel;Gardner, Dale R.;Green, Benedict T.
真菌内生菌（棘豆Undifilum oxytropis）参与了棘豆属和黄芪属物种（通常称为疯草）中苦马豆素的合成。建立了棘豆属和黄芪属植物内生真菌的定量PCR方法。定量限估计为0.2 pg内生菌/ng总DNA。该分析方法用于定量丝光棘豆（白色点疯草）、毛黄芪（羊毛疯草）和雀斑黄芪（斑点疯草）各10株植物中内生菌的量。在所有三个物种的个体之间的内生菌含量观察到一个显着的个体植物变异。在一个O。sericea和A.雀斑病属植物苦马豆素浓度接近或低于检测极限。与其他标本相比，这些植物的内生菌含量也最低。这种方法将是一个有用的工具，在进一步调查的作用，内生菌在苦马豆素生产在各种疯草物种。
A fungal endophyte (Undifilum oxytropis) has been implicated in the synthesis of swainsonine in Oxytropis and Astragalus species, commonly known as locoweeds. A quantitative PCR method has been developed to measure the amount of endophyte in Oxytropis and Astragalus species. The limit of quantitation was estimated to be 0.2 pg of endophyte/ng of total DNA. This method of analysis was used to quantify the amount of endophyte in 10 plants each of Oxytropis sericea (white point locoweed), Astragalus mollissimus (wooly locoweed), and Astragalus lentiginosus (spotted locoweed). A significant amount of individual plant variability was observed in endophyte content among individuals in all three species. In one O. sericea and one A. lentiginosus plant swainsonine concentrations were near or below the limit of detection. These plants also had the lowest amounts of endophyte when compared to the other specimens. This method will be a useful tool in further investigating the role the endophyte plays in swainsonine production in various locoweed species.