PRIMARY SEQUENCE OF RABBIT ALPHA-GLOBIN MESSENGER-RNA
PRIMARY SEQUENCE OF RABBIT ALPHA-GLOBIN MESSENGER-RNA
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DOI：
10.1016/0092-8674(78)90081-8
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
SALSER, WA
中科院分区：
文献类型：
--
作者：
HEINDELL, HC;LIU, A;SALSER, WA
兔子α-嵌合质粒pHb 72中的球蛋白DNA插入（Liu等）采用Maxam和吉尔伯特的方法进行测序。确定了起始密码子前9个核苷酸的5“非翻译区开始并延伸至翻译区前361个核苷酸的mRNA序列。这些数据与Baralle确定的mRNA 5 ''端序列和Proudfoot等人确定的3 ''端序列重叠并完全一致。该序列与先前报道的部分互补[c]RNA测序数据一致（Paddock et al.）并且与公开的氨基酸序列一致。除了由Proudfoot等人确定的3“序列中的3个不确定的核苷酸之外，这项工作标志着兔α-珠蛋白mRNA。基因拷贝可以在体外合成，克隆到细菌质粒中并在宿主中维持的高保真度得到了重申。mRNA核苷酸序列的一般特征进行了讨论，特别注意观察到的碱基组成和密码子偏好，并将该序列与其他完整的mRNA基因序列进行比较。一个新的计算机程序被用来搜索完整的mRNA的最稳定的碱基配对安排。
The rabbit .alpha.-globin DNA insertion in the chimeric plasmid pHb72 (Liu et al.) was sequenced by the method of Maxam and Gilbert. The mRNA sequence beginning in the 5'' untranslated region 9 nucleotides before the initiation codon and extending through the 1st 361 nucleotides of the translated region was determined. The data overlap and are in complete agreement with sequences determined by Baralle for the 5'' end of the mRNA and by Proudfoot et al. for the 3'' end. The sequence is in agreement with the partial complementary[c]RNA sequencing data reported previously (Paddock et al.) and with the published amino acid sequence. Save for 3 uncertain nucleotides in the 3'' sequence determined by Proudfoot et al. this work marks the completion of the primary sequence of the rabbit .alpha.-globin mRNA. The high fidelity with which gene copies can be synthesized in vitro, cloned in a bacterial plasmid and maintained in the host is reaffirmed. The general features of the mRNA nucleotide sequence are discussed with particular attention given to the base composition and codon preferences observed and to comparison of this sequence with other completed mRNA gene sequences. A new computer program was used to search for the most stable base-pairing arrangement of the completed mRNA.