Bioreactors for Guiding Muscle Tissue Growth and Development
Bioreactors for Guiding Muscle Tissue Growth and Development
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DOI：
10.1007/10_2008_2
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Baar, K.
中科院分区：
文献类型：
--
作者：
Dennis, R. G.;Smith, B.;Baar, K.
肌肉组织生物反应器是一种用于指导和监测工程化肌肉组织发育的装置。这些设备的现代历史可以追溯到一个多世纪，因为肌肉组织生物反应器的关键元素已经被研究了很长时间。这些措施包括屏障隔离和细胞、组织和器官在脱离宿主生物体后的培养；提供各种旨在促进生长和维持肌肉的刺激，如电和机械刺激；以及提供灌流液，如培养液或血液衍生物质。只有在这一努力的历史背景下，才能准确地评估我们目前在肌肉生物反应器开发方面的进展。现代的研究更多地集中在使用计算机控制和应用机械应变作为刺激，以及在分离的肌肉细胞培养的早期阶段对底物表面进行修饰以诱导细胞组织。
Muscle tissue bioreactors are devices which are employed to guide and monitor the development of engineered muscle tissue. These devices have a modern history that can be traced back more than a century, because the key elements of muscle tissue bioreactors have been studied for a very long time. These include barrier isolation and culture of cells, tissues and organs after isolation from a host organism; the provision of various stimuli intended to promote growth and maintain the muscle, such as electrical and mechanical stimulation; and the provision of a perfusate such as culture media or blood derived substances. An accurate appraisal of our current progress in the development of muscle bioreactors can only be made in the context of the history of this endeavor. Modern efforts tend to focus more upon the use of computer control and the application of mechanical strain as a stimulus, as well as substrate surface modifications to induce cellular organization at the early stages of culture of isolated muscle cells.