Characterization and Pathogenicity of Colletotrichum gloeosporioides and C. karstii Causing Preharvest Disease on Citrus sinensis in Italy
Characterization and Pathogenicity of Colletotrichum gloeosporioides and C. karstii Causing Preharvest Disease on Citrus sinensis in Italy
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DOI：
10.1111/jph.12299
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发表时间：
2015-03-01
影响因子：
1.5
通讯作者：
Polizzi, Giancarlo
中科院分区：
文献类型：
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作者：
Aiello, Dalia;Carrieri, Raffaele;Polizzi, Giancarlo
2010年至2013年期间，在意大利南部卡塔尼亚、锡拉库萨和恩纳省的6个果园中，检测到不同品种的甜橙出现了采收前症状。共分离到56株单孢菌，经形态和分子鉴定，其中44株为胶孢炭疽菌，12株为卡氏炭疽菌。以ITS1和ITS4为引物，微管蛋白基因特异引物TubGF1和TubGR，3-磷酸甘油醛脱氢酶基因特异引物GDF-GDR为模板，对柑橘炭疽菌分离物进行了鉴定。用BLASTn程序对分离株的ITS1-5.8S-ITS2区、约500bp的微管蛋白基因片段和220bp的GAPDH基因片段进行了测序和分析。Koch的假设是通过对Tarocco Scire‘和Tarocco Nucellare’的果实进行的致病性测试来实现的，其中具有代表性的胶孢子虫和C.karstii菌株。田间调查和致病力测定表明，两种真菌在果实感病能力和致病力上存在显著差异。据我们所知，这是首次报道炭疽菌的出现。在收获前的条件下引起炭疽病。
During the period from 2010 to 2013 preharvest symptoms were detected on different cultivars of sweet orange in six orchards in Catania, Siracusa and Enna provinces, Southern Italy. A total of 56 monosporic fungal isolates were obtained, and among these, 44 were identified as Colletotrichum gloeosporioides and 12 as C.karstii through morphological and molecular analysis. PCR with primers ITS1 and ITS4, primers TubGF1 and TubGR specific for -tubulin gene, primers GDF-GDR, specific for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, were used to confirm the identification of Colletotrichum isolates from citrus. The ITS1-5.8S-ITS2 region, a portion of approximately 500bp of -tubulin gene and a fragment of 220 bp of GAPDH gene of the isolates were sequenced and analysed with the BLASTn program. Koch's postulates were fulfilled by pathogenicity tests carried out on fruit of Tarocco Scire' and Tarocco Nucellare' with representative isolates of C.gloeosporioides and C.karstii. Field surveys and pathogenicity tests revealed significant differences in fruit susceptibility between Tarocco Scire' and Tarocco Nucellare' and in virulence between the fungal species. To our knowledge, this is the first report on the emergence of Colletotrichum spp. causing anthracnose in preharvest conditions.