Simultaneous detection of different glycosidase activities by 19F NMR spectroscopy
Simultaneous detection of different glycosidase activities by 19F NMR spectroscopy
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DOI：
10.1016/s0008-6215(00)00060-4
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发表时间：
2000-08-07
影响因子：
3.1
通讯作者：
Dax, K
中科院分区：
文献类型：
--
作者：
Albert, M;Repetschnigg, W;Dax, K
一种快速的方法，用于同时检测不同的糖苷水解活性在市售的酶制剂和粗培养物中，发现在使用，作为底物，不同的糖基氟化物的混合物和F-19 NMR光谱作为筛选技术。同时，还对这些氟化物在各种缓冲系统中的水解稳定性以及长期储存条件进行了研究。本文给出了一种制备β-D-吡喃甘露糖基氟的简单方法。(C)2000爱思唯尔科技有限公司版权所有。
A fast method for the simultaneous detection of different glycosidolytic activities in commercially available enzyme preparations and crude culture filtrates was found in using, as substrate, a mixture of different glycosyl fluorides and F-19 NMR spectroscopy as a screening technique. Accompanying studies regarding the hydrolytic stability of these fluorides in various buffer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of beta-D-mannopyranosyl fluoride in gram quantities is given. (C) 2000 Elsevier Science Ltd. All rights reserved.