Label-free identification of individual bacteria using Fourier transform light scattering
Label-free identification of individual bacteria using Fourier transform light scattering
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DOI：
10.1364/oe.23.015792
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发表时间：
2015-06-15
期刊：
影响因子：
3.8
通讯作者：
Park, YongKeun
中科院分区：
文献类型：
--
作者：
Jo, YoungJu;Jung, JaeHwang;Park, YongKeun
快速鉴定细菌种类对于医学和食品卫生至关重要。为了在单个细菌水平上实现快速、无标记的细菌物种鉴定，我们提出并实验演示了一种基于傅里叶变换光散射（FTLS）测量和统计分类的光学方法。对于属于四种细菌（单核细胞增生李斯特氏菌、大肠杆菌、干酪乳杆菌和枯草芽孢杆菌）的单个杆状细菌，使用 FTLS 技术精确测量二维角度分辨光散射图。然后系统地分析散射图，采用统计分类来提取每个物种的独特指纹模式，以便可以通过单次光散射测量来识别新的未识别细菌。我们的方法的单一细菌和无标记性质表明其广泛适用于快速护理点细菌诊断。 (C) 2015年美国光学学会
Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on Fourier transform light scattering (FTLS) measurements and statistical classification. For individual rod-shaped bacteria belonging to four bacterial species (Listeria monocytogenes, Escherichia coli, Lactobacillus casei, and Bacillus subtilis), two-dimensional angle-resolved light scattering maps are precisely measured using FTLS technique. The scattering maps are then systematically analyzed, employing statistical classification in order to extract the unique fingerprint patterns for each species, so that a new unidentified bacterium can be identified by a single light scattering measurement. The single-bacterial and label-free nature of our method suggests wide applicability for rapid point-of-care bacterial diagnosis. (C) 2015 Optical Society of America