Centrifugal subfractionation of polymorphonuclear leukocytes, lymphocytes and erythrocytes.
Centrifugal subfractionation of polymorphonuclear leukocytes, lymphocytes and erythrocytes.
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多形核白细胞、淋巴细胞和红细胞的离心亚分级。
DOI：
10.1182/blood.v9.8.804.804
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发表时间：
1954
期刊：
影响因子：
20.3
通讯作者：
D. F. Waugh
中科院分区：
文献类型：
--
作者：
B. Agranoff;B. Vallée;D. F. Waugh
采用一种利用密度差异的方法，将多形性白细胞与淋巴细胞和红细胞分离。在单次离心过程中，淋巴细胞聚集在血浆-白蛋白界面（白蛋白I，密度= 1.065至1.085），多形核白细胞聚集在白蛋白I-白蛋白II界面（白蛋白II，密度= 1.094），红细胞通过Wintrobe管底部。产量超过90%的纯细胞类型。各层均与血液等渗。
The separations of polymorphonuclear leukocytes from lymphocytes and erythrocytes has been accomplished by employing a method which takes advantage of their density differentials. During a single centrifugations the lymphocytes accumulate at a plasma-albumin interface (albumin I, density = 1.065 to 1.085), the polymorphonuclear leukocytes accumulate at an albumin I-albumin II interface (albumin II, density = 1.094) and the erythrocytes pass to the base of the Wintrobe tube. The yields are over 90 per cent pure cell types. All layers are isosmotic with blood.