Effect of addition of hyaluronic acids on the osteoconductivity and biodegradability of synthetic octacalcium phosphate
Effect of addition of hyaluronic acids on the osteoconductivity and biodegradability of synthetic octacalcium phosphate
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DOI：
10.1016/j.actbio.2013.09.005
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发表时间：
2014-01-01
影响因子：
9.7
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Suzuki, Kentaro;Anada, Takahisa;Suzuki, Osamu
本研究旨在研究三种透明质酸钠（HyA）医疗产品Artz（R）、Suvenyl（R）和HyA钠的化学修饰衍生物Synvisc（R）是否可用作骨传导性磷酸八钙（OCP）的合适载体。将OCP颗粒（300-500 μ m直径）与分子量为90 × 104（Artz（R））、190 × 104（Suvenyl（R））和600 × 104（Synvisc（R））的这些HyA钠（分别称为HyA 90、HyA 190和HyA 600）混合。将OCP-HyA复合材料注射到聚四氟乙烯环中，置于小鼠颅骨骨膜下区域3周和6周，然后通过组织形态计量学评估骨形成。TRAP染色法检测HyAs对RANKL诱导RAW 264细胞破骨细胞形成的影响。与单独的OCP相比，OCP与HyA 90和HyA 600的复合物通过增强OCP的骨吸收而增强骨形成。HyA 90和HyA 600促进体外破骨细胞形成。结果表明，HyA/OCP复合材料具有良好的骨传导性能，HyA/OCP复合材料是一种可注射的生物活性骨替代材料。(C)2013 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The present study was designed to investigate whether three sodium hyaluronic acid (HyA) medical products, Artz (R), Suvenyl (R) and a chemically modified derivative of sodium HyA Synvisc (R), can be used as suitable vehicles for an osteoconductive octacalcium phosphate (OCP). OCP granules (300-500 pm diameter) were mixed with these sodium HyAs with molecular weights of 90 x 10(4) (Artz (R)), 190 x 10(4) (Suvenyl (R)) and 600 x 104 (Synvisc (R)) (referred to as HyA90, HyA190 and HyA600, respectively). OCP-HyA composites were injected using a syringe into a polytetrafluoroethylene ring, placed on the subperiosteal region of mouse calvaria for 3 and 6 weeks, and then bone formation was assessed by histomorphometry. The capacity of the HyAs for osteoclast formation from RAW264 cells with RANKL was examined by TRAP staining in vitro. Bone formation was enhanced by the OCP composites with HyA90 and HyA600, compared to OCP alone, through enhanced osteoclastic resorption of OCP. HyA90 and HyA600 facilitated in vitro osteoclast formation. The results suggest that the osteoconductive property of OCP was accelerated by the HyAs-associated osteoclastic resorption of OCP, and therefore that HyA/OCP composites are attractive bone substitutes which are injectable and bioactive materials. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.