Synthetic and Systems Biotechnology
Synthetic and Systems Biotechnology
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合成与系统生物技术
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
F. Calaprice
中科院分区：
文献类型：
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作者：
F. Calaprice
生物体可以通过增强内源性代谢途径或通过引入从其他生物体借用的外源性途径或自然界未知的重新设计的途径来工程化以产生各种各样的化合物。虽然瓶颈酶的过表达和竞争途径的缺失仍然是代谢途径工程的核心，但要成功开发用于生产有价值产品的菌株，还需要考虑许多其他关键因素。这些关键因素包括：宿主生物的选择，必须考虑宿主的天然优势和劣势，以特定的应用;发现，设计和优化代谢途径的计算方法;转录控制工程，以微调酶表达的水平和时间;酶工程，以设计特定代谢途径所需的新的酶活性;开发新的合成生物学工具，以提高基因干预的能力和便利性;以及减少代谢负担的创新战略。这期《合成与系统生物技术》专刊，聚焦于“代谢途径工程”，包括八篇综述文章和五篇原创研究，提供了这些关键要素如何支持和增强代谢工程的优秀例子。
Organisms can be engineered to produce a wide variety of compounds by either enhancing endogenous metabolic pathways, or by introducing exogenous pathways that are either borrowed from other organism, or de novo-designed pathways unknown to nature. While overexpression of bottleneck enzymes and deletion of competing pathways remain at the core of metabolic pathway engineering, there are many other key elements that need to be considered to successfully develop strains for the production of valuable products. Some of these key elements include the choice of host organism, whichmust take into account the natural advantages and disadvantages of the host for a specific application; computational methods to discover, design, and optimize metabolic pathways; transcriptional control engineering, to finetune the levels and timing of enzyme expression; enzyme engineering to design novel enzymatic activities required in specific metabolic pathways; development of new synthetic biology tools to increase the capabilities and expedience of genetic interventions; and innovative strategies to reduce metabolic burden. This special issue of Synthetic and Systems Biotechnology, focusing on “Metabolic Pathway Engineering” comprises eight review articles and five original research studies that provide excellent examples of how these key elements support and augment metabolic engineering.