Giant eosinophil colonies from cultures of bone marrow cells.
Giant eosinophil colonies from cultures of bone marrow cells.
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来自骨髓细胞培养物的巨大嗜酸性粒细胞集落。
DOI：
10.1007/bf02623503
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Weiler,D
中科院分区：
文献类型：
--
作者：
Butterfield,JH;Weiler,D
迄今为止，体外培养嗜酸性粒细胞菌落的小尺寸和生长缓慢阻碍了克隆嗜酸性粒细胞的研究。我们发现，在骨髓细胞甲基纤维素培养中，使用明确补充的牛犊牛血清（DSBCS）与EL4条件培养基（EL4- cm）相结合，嗜酸性粒细胞集落大小和数量增加。在第9、16和23天，与胎牛血清/EL4-CM培养相比，DSBCS/EL4-CM培养的嗜酸性粒细胞集落和细胞/集落均显著增加。在体外产生大量嗜酸性粒细胞的能力有助于克隆嗜酸性粒细胞的研究。
To date, the small size and slow growth of eosinophil colonies in vitro has hampered study of cloned eosinophils. We found enhanced eosinophil colony size and numbers in methylcellulose cultures of bone marrow cells utilizing defined supplemented bovine calf serum (DSBCS) in combination with EL4 conditioned medium (EL4-CM). At days 9, 16 and 23 significantly more eosinophil colonies and more cells/colony were present in cultures incubated with DSBCS/EL4-CM than in cultures incubated with fetal calf serum/EL4-CM. The ability to generate large numbers of eosinophils in vitro should facilitate study of cloned eosinophils.