The fate of paternal mitochondrial DNA in developing female mussels, Mytilus edulis: implications for the mechanism of doubly uniparental inheritance of mitochondrial DNA.
The fate of paternal mitochondrial DNA in developing female mussels, Mytilus edulis: implications for the mechanism of doubly uniparental inheritance of mitochondrial DNA.
复制标题
发育中的雌性贻贝（贻贝）中父本线粒体 DNA 的命运：对线粒体 DNA 双单亲遗传机制的影响。
作者：
B. W. Sutherland;Donald T. Stewart;E. Kenchington;Eleftherios Zouros
海洋贻贝科的物种有两种线粒体DNA：一种是从母亲传给雌性和雄性后代的(F型)，另一种是只从父亲传给儿子的(M型)。通过使用只产生女性后代或雌雄混合后代的配对，以及一对扩增M基因的部分COIII基因而不是F线粒体基因组的寡核苷酸引物，我们证明了男性和女性胚胎都通过精子获得M mtDNA，在受精后24小时内，M mtDNA在女性胚胎中被消除或急剧减少，但在男性胚胎中保持不变。这些观察结果对于理解线粒体DNA双亲遗传物种的线粒体DNA传递和性别决定之间的关系是重要的。
Species of the marine mussel family Mytilidae have two types of mitochondrial DNA: one that is transmitted from the mother to both female and male offspring (the F type) and one that is transmitted from the father to sons only (the M type). By using pair matings that produce only female offspring or a mixture of female and male offspring and a pair of oligonucleotide primers that amplify part of the COIII gene of the M but not the F mitochondrial genome, we demonstrate that both male and female embryos receive M mtDNA through the sperm and that within 24 hr after fertilization the M mtDNA is eliminated or is drastically reduced in female embryos but maintained in male embryos. These observations are important for understanding the relationship between mtDNA transmission and sex determination in species with doubly uniparental inheritance of mitochondrial DNA.