Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.
Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.
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奶牛妊娠早期外周血免疫细胞中干扰素刺激基因和白细胞介素 10 的上调。
DOI：
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发表时间：
2012
影响因子：
1.8
通讯作者：
A. Miyamoto
中科院分区：
文献类型：
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作者：
K. Shirasuna;Haruka Matsumoto;E. Kobayashi;A. Nitta;S. Haneda;M. Matsui;C. Kawashima;K. Kida;Takashi Shimizu;A. Miyamoto
在奶牛中，干扰素-tau（IFNT）在人工授精（AI）后15-19天左右调节母体识别。本研究假设，如果IFNT的关键靶基因在妊娠早期明显上调，则这些基因可用作奶牛未来妊娠的指标。因此，我们确定了这些基因的表达在外周血单核白细胞（PBMC）和多形核粒细胞（PMN）在母体识别期（MRP）。在第0天对20头经产荷斯坦奶牛进行AI，并将其分为以下组：妊娠（Preg，n = 9）、胚胎死亡（艾德，n = 5）和非妊娠（NP，n = 6）。在第12-21天，Preg组的预后水平高于NP组。第8天，Preg组PBMC中的ISG 15和OAS-1（IFN刺激基因：ISG）mRNA高于NP组，第5天，Preg组PMN中的这些mRNA高于NP和艾德组。白细胞介素-10（IL-10，Th 2细胞因子）mRNA的表达增加，在第8天的PBMCs的怀孕母牛。各组肿瘤坏死因子α（TNFα，Th 1细胞因子）mRNA表达均稳定。在体外细胞培养实验中，IFNT刺激PBMC和PMN中ISGs的mRNA表达。IFN-γ刺激PBMC中IL-10 mRNA的表达，而IFN-γ增加PBMC中TNFα mRNA的水平。结果表明，ISGs和IL-10可以响应IFNT之前MRP在外周血免疫细胞，并可能是有用的靶基因的可靠指标妊娠前MRP。
In cows, interferon-tau (IFNT) regulates maternal recognition around days 15-19 after artificial insemination (AI). The present study hypothesized that if key target genes of IFNT are clearly upregulated in earlier stages of pregnancy, these genes could be use as indices of future pregnancy in cows. Therefore, we determined the expression of these genes in peripheral blood mononuclear leukocytes (PBMCs) and polymorphonuclear granulocytes (PMNs) during the maternal recognition period (MRP). Twenty multiparous Holstein cows were subjected to AI on day 0 and categorized into the following groups: pregnancy (Preg, n = 9), embryonic death (ED, n = 5) and non-pregnancy (NP, n = 6). Progesterone levels in the Preg group were higher than those in the NP group on days 12-21. ISG15 and OAS-1 (IFN-stimulated genes: ISGs) mRNA in PBMCs on day 8 was higher in the Preg group than in the NP group, and these mRNAs in PMNs was higher in the Preg group on day 5 than in the NP and ED groups. Interleukin-10 (IL-10, Th2 cytokine) mRNA expression increased on day 8 in the PBMCs of pregnant cows. Tumor necrosis factor α (TNFα, Th1 cytokine) mRNA expression was stable in all groups. In an in vitro cell culture experiment, IFNT stimulated mRNA expression of ISGs in both PBMCs and PMNs. IFNT stimulated IL-10 mRNA expression in PBMCs, whereas IFNT increased TNFα mRNA levels in PBMCs in vitro. The results suggest that ISGs and IL-10 could be responsive to IFNT before the MRP in peripheral blood immune cells and may be useful target genes for reliable indices of pregnancy before the MRP.
DOI：
10.1016/j.anireprosci.2004.04.014
发表时间：
2004-07-05
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
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作者：
Spencer, Thomas E;Bazer, Fuller W
通讯作者：
Bazer, Fuller W