Microfluidic flow-flash: Method for investigating protein dynamics
Microfluidic flow-flash: Method for investigating protein dynamics
复制标题
DOI：
10.1021/ac061193x
复制
发表时间：
2007-01-01
影响因子：
7.4
通讯作者：
Dyer, R. Brian
中科院分区：
文献类型：
--
作者：
Toepke, Michael W.;Brewer, Scott H.;Dyer, R. Brian
我们报道了一种新的方法，微流控流动闪光，用于测量蛋白质反应动力学。该方法将显微镜成像检测系统与微流体流动池耦合以减少数据采集时间和样品消耗。这种组合允许同时收集光谱和时间信息。微流体流动池设计利用三维鞘流来减少样品分散并最小化样品消耗。改变微流体流动池中的流速的能力允许以亚毫秒时间分辨率研究各种时间尺度。成像检测系统可以与多种光谱探针耦合，包括荧光和紫外/可见吸收光谱。在这里，我们利用微流控流动闪光的方法来探测动力学CO重组或O-2结合到肌红蛋白后，激光诱导的光解CO从肌红蛋白的UV/可见光吸收光谱成像。
We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design utilizes three-dimensional sheath flow to reduce sample dispersion and minimize sample consumption. The ability to alter the flow rates in the microfluidic flow cells allows a variety of time scales to be studied with submillisecond time resolution. The imaging detection system can be coupled with several spectroscopic probes including fluorescence and UV/visible absorbance spectroscopy. Here, we utilize the microfluidic flow-flash method to probe the kinetics of CO recombination or O-2 binding to myoglobin after the laser-induced photolysis of CO from myoglobin by UV/visible absorbance spectral imaging.