ORGANIZATION OF INTERNUCLEOSOMAL DNA IN RAT-LIVER CHROMATIN
ORGANIZATION OF INTERNUCLEOSOMAL DNA IN RAT-LIVER CHROMATIN
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DOI：
10.1002/j.1460-2075.1983.tb01379.x
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发表时间：
1983-01-01
期刊：
影响因子：
11.4
通讯作者：
PRUNELL, A
中科院分区：
文献类型：
--
作者：
STRAUSS, F;PRUNELL, A
对经核酸外切酶III和S1核酸酶修剪的核小体二聚体DNA长度分布的详细分析表明，上述大鼠肝脏核小体间距离的变化，至少对于核小体的一个子集来说，是DNA螺旋重复序列的整数倍。用DNase II消化染色质的结果进一步表明，核小体间DNA的长度是DNA螺旋重复序列加上.apprx的整数倍。5个碱基对。讨论了这些特征对核小体沿纤维排列的限制。
A detailed analysis of the length distribution of DNA in nucleosome dimers trimmed with exonuclease III and S1 nuclease suggest that the previously described variation of internucleosomal distance in rat liver occurs, at least for a subset of the nucleosomes, by integral multiples of the helical repeat of the DNA. Results obtained upon digestion of chromatin with DNase II further suggest that lengths of internucleosomal DNA are integral multiples of the helical repeat of the DNA plus .apprx. 5 bp [base pair]. Restraints imposed by these features on the arrangement of nucleosomes along the fiber are discussed.