Extraction of polar and nonpolar biomarkers from the martian soil using aqueous surfactant solutions
Extraction of polar and nonpolar biomarkers from the martian soil using aqueous surfactant solutions
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DOI：
10.1016/j.pss.2012.03.006
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发表时间：
2012-07-01
影响因子：
2.4
通讯作者：
Sephton, Mark A.
中科院分区：
文献类型：
--
作者：
Court, Richard W.;Rix, Catherine S.;Sephton, Mark A.
生命标记芯片的设计是为了探测火星土壤中生命的化学证据。它将使用表面活性剂水溶液从火星土壤中提取极性和非极性生物标记物，并将它们传输到基于抗体的检测器进行表征。目前，1.5gl(-1)聚山梨酯80：20：80(体积：体积)甲醇：水的溶液正在考虑中，似乎是合适的。在这里，我们研究了一系列其他表面活性剂溶液提取火星土壤模拟物JSC MARS-1表面添加的一套八种标准的能力，并测试了最好的两种表面活性剂与具有代表性的抗体分析检测芘的兼容性。结果表明，用20：80(体积比)的甲醇：水作溶剂比单独用水的回收率高。泊洛沙姆表面活性剂Pluronic(R)F-68和Pluronic(R)F-108在这里测试的任何浓度下都不能有效地从JSC MARS-1中提取标准物质。含氟表面活性剂Zonyl(R)FS-300能够提取标准物质，但不如聚山梨酸酯80溶液有效。最成功的替代表面活性剂是聚硅氧烷poly[dimethylsiloxane-co-[3-(2-(2-hydroxyethoxy)ethoxy)propyl]methylsiloxane](PDMSHEPMS)，它能够从JSC MARS-1中提取标准物质，其效率与相同浓度的聚山梨酯80溶液相当。将表面活性剂L的浓度从1.5g L(-1)增加到10g(-1)，可提高聚山梨酯80和PDMSHEPMS溶液对标准物质的回收率，使标准物质的回收率提高约50%。浓度为1.5gl(-1)和10gl(-1)的聚山梨酯80和Zonyl(R)FS-300和PDMSHEPMS(均为10gl(-1)浓度)也与典型的芘抗体检测方法兼容。(C)2012爱思唯尔有限公司。保留所有权利。
The Life Marker Chip is being designed to detect the chemical evidence of life in the martian soil. It will use an aqueous surfactant solution to extract polar and nonpolar biomarkers from the martian soil and to transport them into an antibody-based detector for characterisation. Currently, a solution of 1.5 gl(-1) polysorbate 80 in 20:80 (vol:vol) methanol:water is being considered and appears to be suitable. Here, we have investigated the ability of a range of other surfactant solutions to extract a suite of eight standards spiked on the surfaces of the martian soil simulant JSC Mars-1 and tested the compatibility of the best two surfactants with a representative antibody assay for the detection of pyrene. The results show that using 20:80 (vol:vol) methanol:water as the solvent leads to increased recoveries of standards than using water alone. The poloxamer surfactants Pluronic (R) F-68 and Pluronic (R) F-108 are not effective at extracting the standards from JSC Mars-1 at any of the concentrations tested here. The fluorosurfactant Zonyl (R) FS-300 is able to extract the standards, but not as efficiently as polysorbate 80 solutions. Most successful of the alternative surfactants was the polysiloxane poly[dimethylsiloxane-co-[3-(2-(2-hydroxyethoxy)ethoxy)propyl]methylsiloxane] (PDMSHEPMS) which is able to extract the standards from JSC Mars-1 with an efficiency approximately equal to that of polysorbate 80 solutions of the same concentration. Enhanced recovery of the standards using polysorbate 80 and PDMSHEPMS solutions can be achieved by increasing the concentration of surfactant, from 1.5 g l(-1) to 10 gl(-1), leading to an increase in the recovery of standards of about 50%. Polysorbate 80 at concentrations of 1.5 gl(-1) and 10 gl(-1) and Zonyl (R) FS-300 and PDMSHEPMS (both at a concentration of 10 gl(-1)) are also compatible with the representative pyrene antibody assay. (C) 2012 Elsevier Ltd. All rights reserved.